Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.creatorSánchez Viafara, Jesús Alfonso-
dc.creatorVasconcelos, Gisvani Lopes de-
dc.creatorMaculan, Renata-
dc.creatorAlves, Nadja Gomes-
dc.creatorFerreira, Marcos Brandao Dias-
dc.creatorSudano, Mateus José-
dc.creatorMingoti, Gisele Zoccal-
dc.creatorNunes, Giovana Barros-
dc.creatorLima, Renato Ribeiro de-
dc.creatorDrumond, Roberti Martins-
dc.creatorSantos, Raphael Nunes dos-
dc.creatorEberlin, Marcos Nogueira-
dc.creatorNegrão, Fernanda-
dc.creatorDonato, Mariana Aragão M.-
dc.creatorPeixoto, Christina A.-
dc.creatorSouza, José Camisão de-
dc.identifier.citationSÁNCHEZ VIAFARA, J. A. et al. Peroxisome proliferator-activated receptor delta-PPARδ agonist (L-165041) enhances bovine embryo survival and post vitrification viability. Reproduction, Fertility and Development, Melbourne, v. 34, n. 9, p. 658-668, 2022. DOI: 10.1071/RD21245.pt_BR
dc.description.abstractThe effect of L-165041 (PPARδ-agonist) on decreasing apoptosis and intracellular lipid content was assessed in fresh and vitrified–warmed in vitro-produced bovine embryos. It was hypothesised that the addition of L-165041 to the culture medium enhances development and cryopreservation. Oocytes were allocated to one of two treatments: control-standard culture medium, or L-165041 added to the medium on day 1 with no media change. Ultrastructure, cleavage, and blastocyst rates were evaluated in fresh, and in post-vitrification cultured embryos by optical and electronic microscopy. A subset of fresh embryos were fixed for TUNEL assay and for Sudan-Black-B histochemical staining. Vitrified–warmed embryos were assessed using MALDI-MS technique. Cleavage and blastocyst rates (control 49.4 ± 5.2, L-165041 51.8 ± 4.3) were not influenced by L-165041. The proportion of inner cell mass cells (ICM) was higher in fresh embryos, and the rate of total and ICM apoptosis was lower in L-165041. In warmed-embryos, total and ICM apoptosis was lower in L-165041. The overall hatching rate was higher in L-165041 (66.62 ± 2.83% vs 53.19 ± 2.90%). There was less lipid accumulation in fresh L-165041-embryos. In conclusion, the use of L-165041 is recommended to improve the viability of in vitro-derived bovine embryos.pt_BR
dc.publisherCSIRO Publishingpt_BR
dc.sourceReproduction, Fertility and Developmentpt_BR
dc.subjectBovine embryo - Cryopreservationpt_BR
dc.subjectCulture mediumpt_BR
dc.subjectEmbryo apoptosispt_BR
dc.subjectEmbriões bovinos - Criopreservaçãopt_BR
dc.subjectMeio de culturapt_BR
dc.subjectApoptose do embriãopt_BR
dc.titlePeroxisome proliferator-activated receptor delta-PPARδ agonist (L-165041) enhances bovine embryo survival and post vitrification viabilitypt_BR
Appears in Collections:DES - Artigos publicados em periódicos
DEX - Artigos publicados em periódicos

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.