Please use this identifier to cite or link to this item:
metadata.artigo.dc.title: Banana leaf anatomy characteristics related to ploidy levels
metadata.artigo.dc.creator: Madail, Rafael Hansen
Pio, Leila Aparecida Sales
Rezende, Renata Alves Lara Silva
Pasqual, Moacir
Silva, Sebastião de Oliveira e
metadata.artigo.dc.subject: Musa sp.
Leaf internal morphology
Genetic diversity
Plant microtechnique
metadata.artigo.dc.publisher: Universidade Estadual de Maringá (UEM) 29-Jun-2022
metadata.artigo.dc.identifier.citation: MADAIL, R. H. et al. Banana leaf anatomy characteristics related to ploidy levels. Acta Scientiarum-Agronomy, [S.l.], v. 44, p. 1-8, Jan./Dec. 2022. DOI: 10.4025/actasciagron.v44i1.55709.
metadata.artigo.dc.description.abstract: Many important crops have cultivars with different ploidy and provide a diverse choice of materials for breeding programs. Therefore, it is essential to accurately assess the ploidy of all breeding materials. Increased DNA content is known to have various effects on external and internal morphology, an effect known as the “nucleotypic effect”. Thus, anatomical assessment can be used as a tool for determining ploidy in plants, making the chromosome counting technique or flow cytometry unnecessary. This study aimed to evaluate the leaf anatomical characteristics of banana cultivars and understand the relationship between these features and ploidy levels. Thirteen accessions were evaluated, including diploid, triploid, and tetraploid genotypes, and cultivars, resulting from in vitro propagation after 90 days of acclimatization. Five fully expanded young leaves were collected from each cultivar, fixed in FAA70 (formaldehyde–acetic acid–ethanol) and preserved in 70% alcohol. Transverse and paradermal sections of the abaxial and adaxial regions were taken, and variables such as size and stomatal density, leaf thickness in the midrib and fourth vascular bundle region, and thickness of the epidermis, hypodermis, and parenchyma were measured. Results for leaf thickness, stomatal size, and density proved to be appropriate parameters for characterizing banana ploidy levels.
metadata.artigo.dc.language: en_US
Appears in Collections:DAG - Artigos publicados em periódicos

Files in This Item:
File Description SizeFormat 
ARTIGO_Banana leaf anatomy characteristics related to ploidy levels.pdf308,76 kBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons