Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/57120
Título: First evidence of ehrlichia minasensis infection in horses from Brazil
Palavras-chave: Horse
Ehrlichiosis
Ticks
PCR
IFA
Polymerase Chain Reaction (PCR)
Indirect fluorescent antibody test
Data do documento: 25-Jan-2021
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
Citação: MURARO, L. S. et al. First evidence of ehrlichia minasensis infection in horses from Brazil. Pathogens, [S.l.], v. 10, n. 3, p. 1-11, 2021. DOI: 10.3390/pathogens10030265.
Resumo: The genus Ehrlichia includes tick-borne bacterial pathogens affecting humans, domestic and wild mammals. Ehrlichia minasensis has been identified in different animal species and geographical locations, suggesting that this is a widely distributed and generalist Ehrlichia. In the present study, we evaluated Ehrlichial infection in 148 Equidae presented to the Medical Clinic Department of a Veterinary Hospital from a midwestern region of Brazil. Blood samples and ticks collected from the animals were tested by Polymerase Chain Reaction (PCR) for the presence of Ehrlichia spp. A multigenic approach including Anaplasmataceae-specific (i.e., 16S rRNA, groEL, gltA) and Ehrlichia-specific (i.e., dsb and trp36) genes was used for accurate bacteria identification. Sera samples were also collected and evaluated for the detection of anti-Ehrlichia antibodies by indirect fluorescent antibody test (IFA). Possible associations between molecular and serological diagnostics and clinical and hematological manifestations were tested using chi-squared or Fisher’s exact tests. Sequence analysis of the dsb fragment revealed that three horses (2.03%) were exposed to E. minasensis. Sixty-one (41.2%) Equidae (58 equines and three mules), were seropositive for Ehrlichia spp., with antibody titers ranging between 40 and 2560. Seropositivity to ehrlichial antigens was statistically associated with tick infestation, rural origin, hypoalbuminemia and hyperproteinemia (p ≤ 0.05). The present study reports the first evidence of natural infection by E. minasensis in horses from Brazil.
URI: http://repositorio.ufla.br/jspui/handle/1/57120
Aparece nas coleções:DMV - Artigos publicados em periódicos

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