Buscar

 

RI UFLA (Universidade Federal de Lavras) >
DCS - Departamento de Ciência do Solo >
DCS - Artigos publicados em periódicos >

Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/859

Title: Activation energy of extracellular enzymes in soils from different biomes
???metadata.dc.creator???: Steinweg, J. Megan et al.
Keywords: Bioquímica do Solo
Enzymes
Publisher: Oak Ridge Nat. Lab e outras
Issue Date: 2013
Citation: STEINWEG, J. M. Activation energy of extracellular enzymes in soils from different biomes. PLos One, San Francisco, v. 8, n 3, e59943, Mar. 2013
Abstract: Enzyme dynamics are being incorporated into soil carbon cycling models and accurate representation of enzyme kinetics is an important step in predicting belowground nutrient dynamics. A scarce number of studies have measured activation energy (Ea) in soils and fewer studies have measured Ea in arctic and tropical soils, or in subsurface soils. We determined the Ea for four typical lignocellulose degrading enzymes in the A and B horizons of seven soils covering six different soil orders. We also elucidated which soil properties predicted any measurable differences in Ea. b-glucosidase, cellobiohydrolase, phenol oxidase and peroxidase activities were measured at five temperatures, 4, 21, 30, 40, and 60 C. Ea was calculated using the Arrhenius equation. b-glucosidase and cellobiohydrolase Ea values for both A and B horizons in this study were similar to previously reported values, however we could not make a direct comparison for B horizon soils because of the lack of data. There was no consistent relationship between hydrolase enzyme Ea and the environmental variables we measured. Phenol oxidase was the only enzyme that had a consistent positive relationship between Ea and pH in both horizons. The Ea in the arctic and subarctic zones for peroxidase was lower than the hydrolases and phenol oxidase values, indicating peroxidase may be a rate limited enzyme in environments under warming conditions. By including these six soil types we have increased the number of soil oxidative enzyme Ea values reported in the literature by 50%. This study is a step towards better quantifying enzyme kinetics in different climate zones.
URI: http://repositorio.ufla.br/jspui/handle/1/859
???metadata.dc.language???: en_US
Appears in Collections:DCS - Artigos publicados em periódicos

Files in This Item:

File Description SizeFormat
ARTIGO_Activation energy of extracellular enzymes in soils from392.61 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.


View Statistics

 


DSpace Software Copyright © 2002-2010  Duraspace - Feedback