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|Título: ||Simultaneous analysis of 25 phenolic compounds in grape juice for HPLC: method validation and characterization of São Francisco Valley samples|
|Autor(es): ||Natividade, M. M. P.|
Corrêa, L. C.
Souza, S. V. C.
Pereira, G. E.
Lima, L. C. O.
|Assunto: ||In-house method validation|
|Data de publicação: ||15-Ago-2013|
|Referência: ||NATIVIDADE, M. M. P. et al. Simultaneous analysis of 25 phenolic compounds in grape juice for HPLC: method validation and characterization of São Francisco Valley samples. Microchemical Journal, [S.l.], v. 110, p. 665-674, Sept. 2013.|
|Abstract: ||A HPLC method for simultaneous determination of 25 phenolic compounds in grape juice was optimized, validated
and applied in the characterization of juices produced in São Francisco Valley (SFV), Brazil. The performance char-
acteristics of the method were established by assays with standard solutions of phenolic compounds, spiked and
unspiked samples. Linearity, matrix effects, trueness, precision, detection and quantification limits were evaluated.
Linearity was demonstrated in the concentration ranges tested for all phenolic compounds. Significant matrix ef-
fects were not identified for the studied compounds. Mean recoveries ranged from 86.18 to 106.50%, demonstrat-
ing no lack of trueness. Precision of the method was confirmed for the 25 phenolic compounds, with acceptable
repeatability relative standard deviations (from 0.71 to 9.24%) and within-reproducibility relative standard devia-
tions (from 1.34 to 9.26%) for unspiked and spiked samples. The theoretical limits of detection and quantification of
the method varied from 0.001 to 0.19 μg mL −1 and 0.003 to 0.37 μg mL −1 , respectively. The results of the valida-
tion process showed that the proposed method is fitness for purpose. This method was able to identify simulta-
neously 25 phenolic compounds and had advantages such as low consumption of solvents and easy sample
preparation. The phenolic profile of the grape juices from SFV varied according the grape cultivar. Phenolics of
the anthocyanins and tannins class predominated in red grape juices, while in white grape juice phenolic acids
and tannins were found at high concentrations.|
|Aparece nas coleções: ||DCA - Artigos publicados em periódicos|
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