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|Title: ||Identification of fungi of the genus Aspergillus section nigri using polyphasic taxonomy|
|???metadata.dc.creator???: ||Silva, Daiani M.|
Batista, Luís R.
Rezende, Elisângela F.
Fungaro, Maria Helena P.
|Keywords: ||Polyphasic taxonomy|
Aspergillus spp morphology
|Publisher: ||Sociedade Brasileira de Microbiologia|
|Issue Date: ||13-Jan-2011|
|Citation: ||SILVA, D. M. et al. Identification of fungi of the genus Aspergillus section nigri using polyphasic taxonomy. Brazilian Journal of Microbiology, São Paulo, v. 42, n. 2, p. 761-773, Apr. 2011.|
|Abstract: ||In spite of the taxonomy of the Aspergillus species of the Nigri Section being regarded as troublesome, a
number of methods have been proposed to aid in the classification of this Section. This work aimed to
distinguish Aspergillus species of the Nigri Section from foods, grains and caves on the basis in Polyphasic
Taxonomy by utilizing morphologic and physiologic characters, and sequencing of ß-tubulin and calmodulin
genes. The morphologic identification proved useful for some species, such as A. carbonarius and
Aspergillus sp UFLA DCA 01, despite not having been totally effective in elucidating species related to A.
niger. The isolation of the species of the Nigri Section on Creatine Sucrose Agar (CREA) enabled to
distinguish the Aspergillus sp species, which was characterized by the lack of sporulation and by the
production of sclerotia. Scanning Electron microscopy (SEM) allowed distinguishing the species into two
distinct groups. The production of Ochratoxin A (OTA) was only found in the A. carbonarius and A. niger
species. The sequencing of -tubulin gene was efficient in differing most of the Aspergillus species from the
Nigri Section with the exception of Aspergillus UFLA DCA 01, which could not be distinguished from A.
costaricaensis. This species is morphologically similar to A. costaricaencis for its low sporulation capacity
and high sclerotia production, but it differs morphologically from A. costaricaensis for its conidial
ornamentation and size of vesicles. Equally, based on partial calmodulin gene sequence data Aspergillus
UFLA DCA 01 differs from A. costaricaensis.|
|Appears in Collections:||DCA - Artigos publicados em periódicos|
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