Buscar

 

RI UFLA (Universidade Federal de Lavras) >
DCA - Departamento de Ciência dos Alimentos >
DCA - Artigos publicados em periódicos >

Por favor, utilize esse identificador para citar este item ou usar como link: http://repositorio.ufla.br/jspui/handle/1/9714

Título: Identification of fungi of the genus Aspergillus section nigri using polyphasic taxonomy
Autor(es): Silva, Daiani M.
Batista, Luís R.
Rezende, Elisângela F.
Fungaro, Maria Helena P.
Sartori, Daniele
Alves, Eduardo
Assunto: Polyphasic taxonomy
β-tubulin gene
Aspergillus spp morphology
Publicador: Sociedade Brasileira de Microbiologia
Data de publicação: 13-Jan-2011
Referência: SILVA, D. M. et al. Identification of fungi of the genus Aspergillus section nigri using polyphasic taxonomy. Brazilian Journal of Microbiology, São Paulo, v. 42, n. 2, p. 761-773, Apr. 2011.
Abstract: In spite of the taxonomy of the Aspergillus species of the Nigri Section being regarded as troublesome, a number of methods have been proposed to aid in the classification of this Section. This work aimed to distinguish Aspergillus species of the Nigri Section from foods, grains and caves on the basis in Polyphasic Taxonomy by utilizing morphologic and physiologic characters, and sequencing of ß-tubulin and calmodulin genes. The morphologic identification proved useful for some species, such as A. carbonarius and Aspergillus sp UFLA DCA 01, despite not having been totally effective in elucidating species related to A. niger. The isolation of the species of the Nigri Section on Creatine Sucrose Agar (CREA) enabled to distinguish the Aspergillus sp species, which was characterized by the lack of sporulation and by the production of sclerotia. Scanning Electron microscopy (SEM) allowed distinguishing the species into two distinct groups. The production of Ochratoxin A (OTA) was only found in the A. carbonarius and A. niger species. The sequencing of -tubulin gene was efficient in differing most of the Aspergillus species from the Nigri Section with the exception of Aspergillus UFLA DCA 01, which could not be distinguished from A. costaricaensis. This species is morphologically similar to A. costaricaencis for its low sporulation capacity and high sclerotia production, but it differs morphologically from A. costaricaensis for its conidial ornamentation and size of vesicles. Equally, based on partial calmodulin gene sequence data Aspergillus UFLA DCA 01 differs from A. costaricaensis.
URI: http://repositorio.ufla.br/jspui/handle/1/9714
Idioma: en
Aparece nas coleções: DCA - Artigos publicados em periódicos

Arquivos neste Item:

Arquivo Descrição TamanhoFormato
19-Identification of fungi of the genus Aspergillus section.pdf2,13 MBAdobe PDFVer/abrir

Este item está licenciado com Licença Creative Commons
Creative Commons

Itens protegidos por copyright, com todos os direitos reservados, Salvo indicação em contrário.


Mostrar estatísticas

 


DSpace Software Copyright © 2002-2007 MIT and Hewlett-Packard - Feedback