Mycorrhization stimulant based in formononetin associated to fungicide and doses of phosphorus in soybean in the cerrado

Abstract

Arbuscular mycorrhizal fungi (AMF) are obligatory biotrophics, which complicates the feasibility of producing inoculants. Thus, the discovery of substances capable of stimulating mycorrhizal colonization, like the isoflavone formononetin emerges as a promising alternative to explore the benefits of AMF native soil in extensive crops system. The aim of this study was evaluate the application of the isoflavone formononetin (7-hydroxy-4'-methoxy isoflavone) andfungicide via seed in Cerrado soil in no-tillage system and fertilization with two doses of phosphorus in soybean under field and controlled conditions. In the field experiment the treatments were three levels of formononetin (0, 0.5 and 1.0 mg Formononetin seed-1) in the absence and presence of fungicide (Carbendazim + Thiram commercial mix) applied to seeds with two doses of phosphorus (100 % and 33% of the recommended fertilization). The study was conducted until the maturation of soybeans. The greenhouse conditions experiment was implemented with the same treatments used in the field research and the flowering stage of the soybean plant was simulated water stress during 10 days, returning to normal irrigation until to harvest the soybean grain. Applying of formononetin stimulated the increased of mycorrhizal colonization, number of nodules, vegetative growth and soybean production in the greenhouse. In the field research the treatments contributed to attenuate the negative effect of the fungicide Carbendazim + Thiram in soybean reflecting increased soybean production and adequate supply of soil phosphorus besides high density of propagules of natives AMF that contributed to reduce the benefits of stimulating mycorrhizal (formononetin) in this research