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dc.creatorBadjatia, Nitika-
dc.creatorAmbrósio, Daniela L.-
dc.creatorLee, Ju Huck-
dc.creatorGünzl, Arthur-
dc.date.accessioned2017-09-04T20:09:35Z-
dc.date.available2017-09-04T20:09:35Z-
dc.date.issued2013-05-
dc.identifier.citationBADJATIA, N. et al. Trypanosome cdc2-related kinase 9 controls spliced leader RNA cap4 methylation and phosphorylation of RNA polymerase II subunit RPB1. Mollecular and Cellular Biology, [S. l.], v. 33, n. 10, p. 1965-1975, May 2013.pt_BR
dc.identifier.urihttp://mcb.asm.org/content/33/10/1965.full.pdf+htmlpt_BR
dc.identifier.urirepositorio.ufla.br/jspui/handle/1/15355-
dc.description.abstractConserved from yeast to mammals, phosphorylation of the heptad repeat sequence Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7 in the carboxy-terminal domain (CTD) of the largest RNA polymerase II (RNA Pol II) subunit, RPB1, mediates the enzyme's promoter escape and binding of RNA-processing factors, such as the m7G capping enzymes. The first critical step, Ser5 phosphorylation, is carried out by cyclin-dependent kinase 7 (CDK7), a subunit of the basal transcription factor TFIIH. Many early-diverged protists, such as the lethal human parasite Trypanosoma brucei, however, lack the heptad repeats and, apparently, a CDK7 ortholog. Accordingly, characterization of trypanosome TFIIH did not identify a kinase component. The T. brucei CTD, however, is phosphorylated and essential for transcription. Here we show that silencing the expression of T. brucei cdc2-related kinase 9 (CRK9) leads to a loss of RPB1 phosphorylation. Surprisingly, this event did not impair RNA Pol II transcription or cotranscriptional m7G capping. Instead, we observed that CRK9 silencing led to a block of spliced leader (SL) trans splicing, an essential step in trypanosome mRNA maturation, that was caused by hypomethylation of the SL RNA's unique cap4.pt_BR
dc.description.urihttp://repositorio.ufla.br/jspui/handle/1/15355-
dc.languageen_USpt_BR
dc.publisherNational Institutes of Healthpt_BR
dc.rightsrestrictAccesspt_BR
dc.sourceMollecular and Cellular Biologypt_BR
dc.subjectRNA polymerasept_BR
dc.subjectMethylationpt_BR
dc.subjectPhosphorylationpt_BR
dc.subjectCarboxy-terminal domainpt_BR
dc.subjectMetilaçãopt_BR
dc.subjectFosforilaçãopt_BR
dc.subjectDomínio carboxi-terminalpt_BR
dc.titleTrypanosome cdc2-related kinase 9 controls spliced leader RNA cap4 methylation and phosphorylation of RNA polymerase II subunit RPB1pt_BR
dc.typeArtigopt_BR
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