Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/28269
Registro completo de metadados
Campo DCValorIdioma
dc.creatorNascimento, Vivian Elias-
dc.creatorVon Pinho, Edila Vilela de Resende-
dc.creatorVon Pinho, Renzo Garcia-
dc.creatorNascimento Júnior, A. D. do-
dc.date.accessioned2017-12-15T12:45:29Z-
dc.date.available2017-12-15T12:45:29Z-
dc.date.issued2012-
dc.identifier.citationNASCIMENTO, V. E. et al. Detection limits of the strip test and PCR for genetically modified corn in Brazil. Genetics and Molecular Research, Ribeirão Preto, v. 11, n. 3, p. 2497-2505, 2012.pt_BR
dc.identifier.urihttps://www.geneticsmr.com/articles/detection-limits-of-the-strip-test-and-pcr-for-genetically-modified-corn-in-brazil.pdfpt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/28269-
dc.description.abstractBrazilian legislation establishes a labeling limit for products that contain more than 1% material from genetically modified organisms (GMOs). We assessed the sensitivity of the lateral flow strip test in detection of the GMO corn varieties Bt11 and MON810 and the specificity and sensitivity of PCR techniques for their detection. For the strip test, the GMO seeds were mixed with conventional seeds at levels of 0.2, 0.4 and 0.8% for Bt11, and 0.4, 0.8 and 1.6% for MON810. Three different methodologies were assessed and whole seeds, their endosperm and embryonic axis were used. For the PCR technique, the GMO seeds of each of the two varieties were mixed with conventional seeds at levels of 20, 10, 5, 2, 1, and 0.5%. The seeds were ground and the DNA extracted. For detection of the GMO material, specific primers were used for MON810 and Bt11 and maize zein as an endogenous control. The sensitivity of the strip test varied for both maize varieties and methodologies. The test was positive for Bt11 only at 0.8%, in contrast with the detection limit of 0.4% indicated by the manufacturer. In the multiplex PCR, the primers proved to be specific for the different varieties. These varieties were detected in samples with one GMO seed in 100. Thus, this technique proved to be efficient in detecting contaminations equal to or greater than 1%.pt_BR
dc.languageen_USpt_BR
dc.publisherFundação de Pesquisas Científicas de Ribeirão Pretopt_BR
dc.rightsrestrictAccesspt_BR
dc.sourceGenetics and Molecular Researchpt_BR
dc.subjectTrip testpt_BR
dc.subjectPCRpt_BR
dc.subjectGenetically modified organismpt_BR
dc.titleDetection limits of the strip test and PCR for genetically modified corn in Brazilpt_BR
dc.typeArtigopt_BR
Aparece nas coleções:DAG - Artigos publicados em periódicos

Arquivos associados a este item:
Não existem arquivos associados a este item.


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.