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dc.creatorCarvalho, Milene Alves de Figueiredo-
dc.creatorPaiva, Renato-
dc.creatorStein, Vanessa Cristina-
dc.creatorHerrera, Raírys Cravo-
dc.creatorPorto, Jorge Marcelo Padovani-
dc.creatorVargas, Daiane Peixoto-
dc.creatorAlves, Eduardo-
dc.date.accessioned2018-02-08T12:35:27Z-
dc.date.available2018-02-08T12:35:27Z-
dc.date.issued2014-
dc.identifier.citationCARVALHO, M. A. de F. et al. Induction and morpho-ultrastructural analysis of organogenic calli of a wild passionfruit. Brazilian Archives of Biology and Technology, Curitiba, v. 57, n. 6, p. 851-859, Nov. /Dec. 2014.pt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/28551-
dc.description.abstractThis work studied a new protocol for organogenic calli induction and characterization of the morphology and ultrastructure of callogenesis in leaf explants of Passiflora gibertii N. E. Brown, a native passion fruit species from Brazil. Calli induction was performed in different growth conditions (light and dark), different MS medium salt concentrations (MS and MS half strength) and the presence or absence of coconut water. The leaf explants maintained in the dark were more responsive to bud formation. In order to reduce spending on in vitro culture, the most suitable induction medium for P. gibertii organogenesis could, therefore be the MS half strength salt concentration medium maintained in the dark. The addition of coconut water to the culture medium was essential for both calli induction and bud formation. The morphological and ultrastructural features of the organogenic calli were isodiametric cells, characterized by an organized cellular system, nucleus with prominent nucleoli, presence of starch grains and dense cytoplasm rich in endoplasmic reticulum. The scanning electron microscopy demonstrated that buds were present on these calli.pt_BR
dc.languageen_USpt_BR
dc.publisherInstituto de Tecnologia do Paraná - Tecparpt_BR
dc.rightsAttribution-NonCommercial 4.0 International*
dc.rightsacesso abertopt_BR
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/*
dc.sourceBrazilian Archives of Biology and Technologypt_BR
dc.subjectPassifloraceaept_BR
dc.subjectCallogenesispt_BR
dc.subjectElectromicroscopypt_BR
dc.subjectOptic microscopept_BR
dc.subjectTissue culturept_BR
dc.titleInduction and morpho-ultrastructural analysis of organogenic calli of a wild passionfruitpt_BR
dc.typeArtigopt_BR
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