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dc.creatorDias, Gabrielen de Maria Gomes-
dc.creatorSoares, Joyce Dória Rodrigues-
dc.creatorPasqual, Moacir-
dc.creatorSilva, Renata Alves Lara-
dc.creatorRodrigues, Luiz Carlos de Almeida-
dc.creatorPereira, Fabricio José-
dc.creatorCastro, Evaristo Mauro de-
dc.date.accessioned2018-02-21T16:35:38Z-
dc.date.available2018-02-21T16:35:38Z-
dc.date.issued2014-08-
dc.identifier.citationDIAS, G. de M. G. et al. Photosynthesis and leaf anatomy of Anthurium cv. Rubi plantlets cultured in vitro under different silicon (Si) concentrations. Australian Journal of Crop Science, [S. l.], v. 8, n. 8, p. 1160-1167, Aug. 2014.pt_BR
dc.identifier.urihttp://www.cropj.com/dias_8_8_2014_1160_1167.pdfpt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/28617-
dc.description.abstractThe silicon can induce beneficial changes in plants, such as the further development of tissues and increased photosynthetic rate. Thus, studies on the anatomical changes resulting from in vitroculture are key to better understanding the development of micropropagated plants. Therefore, this study was undertaken to evaluate the morphological and physiological differences in plants with the use of silicon added to the medium for the in vitro culture of Anthurium adreaenum cv. Rubi. Nodal segments of seedlings were established in vitro and inoculated in Pierik medium supplemented with 30 g L-1 sucrose and solidified with 1.8 g L-1 PhytagelTM. Different concentrations of sodium silicate (Na2SiO3)(0.0, 0.5, 1.0 and 2.0 mg L-1) were added to the medium. The experimental design was completely randomized with 30 repetitions. The segments were maintained for 100 days in a growth chamber under controlled conditions and evaluated anatomically and scanning electron microscopy (ultrastructurally) and for their photosynthetic capacities. Medium containing 1.0 mg L-1 sodium silicate promoted the development of higher stomatal densities on the sheets. For the polar (31.38 μm) and equatorial (31.33 μm) diameter of the stomata of the abaxial leaf, the highest averages occurred in the treatment with 2.0 mg L-1. Greater relative polar and equatorial diameters were estimated with a peak concentration of 1.2 mg L-1. The increase in the sodium silicate concentration led to thinning of the abaxial and adaxial epidermis. The thickness of the central rib had a sharp decrease up to 1.3 mg L-1. For the mesophyll, the control displayed a higher thickness, whereas the addition of sodium silicate to the culture medium promoted a decrease. Seedlings grown in sodium silicate displayed significant differences, with increased photosynthetic and transpiration rates, stomatal conductance and internal CO2 concentrations. As for the ratio between the internal and external concentrations of CO2, no significant differences were observed. The addition of sodium silicate resulted in increased epicuticular wax deposition and the formation of structures reserved for depositing calcium. Therefore, under in vitroconditions, the addition of sodium silicate to the culture medium affected the photosynthesis and leaf anatomy of A.andraeanum cv. Rubi, developing anatomical and physiological characteristics that contributed to the survival ex vitro.pt_BR
dc.languageen_USpt_BR
dc.publisherSouthern Cross Publishingpt_BR
dc.rightsrestrictAccesspt_BR
dc.sourceAustralian Journal of Crop Sciencept_BR
dc.subjectAnthuriums – Effect of siliconpt_BR
dc.subjectAnthuriums – Anatomypt_BR
dc.subjectAnthuriums – Physiologypt_BR
dc.subjectIn vitro culturept_BR
dc.subjectMicropropagationpt_BR
dc.subjectAntúrio – Efeito do silíciopt_BR
dc.subjectAntúrio – Anatomiapt_BR
dc.subjectAntúrio – Fisiologiapt_BR
dc.subjectCultivo in vitropt_BR
dc.subjectMicropropagaçãopt_BR
dc.subjectAnthurium adreaenumpt_BR
dc.titlePhotosynthesis and leaf anatomy of Anthurium cv. Rubi plantlets cultured in vitro under different silicon (Si) concentrationspt_BR
dc.typeArtigopt_BR
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