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dc.creatorCarvalho, André Luiz de Andrade-
dc.creatorRezende, Larissa Castro de-
dc.creatorCosta, Lúcio Bertoldo-
dc.creatorHalfeld-Vieira, Bernardo de Almeida-
dc.creatorPinto, Zayame Vegette-
dc.creatorMorandi, Marcelo Augusto Boechat-
dc.creatorMedeiros, Flávio Henrique Vasconcelos de-
dc.creatorBettiol, Wagner-
dc.date.accessioned2018-10-04T13:51:16Z-
dc.date.available2018-10-04T13:51:16Z-
dc.date.issued2018-03-
dc.identifier.citationCARVALHO, A. L. de A. et al. Optimizing the mass production of Clonostachys rosea by liquid-state fermentation. Biological Control, [S.l.], v. 118, p. 16-25, Mar. 2018.pt_BR
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S1049964417302505#!pt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/30903-
dc.description.abstractOptimization of the culture medium and the entire mass production process for various fungi in liquid medium has been studied. However, the technology is not well developed for Clonostachys rosea, a biocontrol agent against various plant pathogens due to its antagonistic capacity to act as a hyperparasite, compete for nutrients and space, and induce plant resistance to pathogens. In this study, we aimed to optimize the culture medium and to standardize parameters that may interfere with the production of C. rosea conidia in a liquid-state fermentation system. Culturing was performed in 250-mL Erlenmeyer flasks shaken for 7 days, followed by planned experimental methodology to reduce the number of analyses and consumable costs. Benchtop bioreactor tests with the optimized medium were performed. Glucose and sucrose were evaluated as carbon sources. Initially, the effects of temperature, pH, photoperiod, carbon:nitrogen ratio and water activity on inoculum production were evaluated, with the pH and photoperiod being factors that contributed to conidial production. Optimization of the fermentation conditions was performed using a central composite rotational design (CCD) with a wider range of pH values and photoperiods. The remaining variables were fixed according to the previous assay. Colony-forming unit (CFUs), biomass production and conidial viability were evaluated, and glucose was used as a carbon source to enhance conidial production. The optimized conditions that resulted in a maximum yield of conidia (1.78 × 107 conidia mL−1), dried biomass (0.558 g) and CFUs (5.15 × 106 CFUs mL−1) were a pH value of 4 and a photoperiod of 12 h.pt_BR
dc.languageen_USpt_BR
dc.publisherElsevierpt_BR
dc.rightsrestrictAccesspt_BR
dc.sourceBiological Controlpt_BR
dc.subjectClonostachys roseapt_BR
dc.subjectMass productionpt_BR
dc.subjectLiquid mediapt_BR
dc.subjectBioreactorpt_BR
dc.subjectFungal productionpt_BR
dc.titleOptimizing the mass production of Clonostachys rosea by liquid-state fermentationpt_BR
dc.typeArtigopt_BR
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