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metadata.artigo.dc.title: Prototheca blaschkeae subsp. brasiliensis subsp. nov., isolated from cow milk
metadata.artigo.dc.creator: Morandi, Stefano
Cremonesi, Paola
Povolo, Milena
Capra, Emanuele
Silvetti, Tiziana
Castiglioni, Bianca
Ribeiro, Márcio Garcia
Alves, Ana Carolina
Costa, Geraldo Márcio da
Luini, Mario
Brasca, Milena
metadata.artigo.dc.subject: Prototheca blaschkeae subsp. brasiliensis
Cow milk
Protothecal bovine mastitis
metadata.artigo.dc.publisher: Society for General Microbiology 2017
metadata.artigo.dc.identifier.citation: MORANDI, S. et al. Prototheca blaschkeae subsp. brasiliensis subsp. nov., isolated from cow milk. International Journal of Systematic and Evolutionary Microbiology, [S.l.], v. 67, n. 10, 3865-3871, 2017.
metadata.artigo.dc.description.abstract: A strain of an achlorophyllic alga, named PR24T, was isolated from cow milk samples from the state of Minas Gerais, Brazil. Based on 18S rDNA, 28S rRNA, D1/D2 region of the LSU rDNA and SSU rRNA gene sequence similarities, this strain was found to be a member of the genus Prototheca and closely related to Prototheca blaschkeae SAG2064T. However, the novel strain could easily be distinguished from recognized Prototheca species by internal transcribed spacer, species-specific PCR, single-strand conformation polymorphism-PCR analysis and phenotypic characteristics. The inability to grow in Sabouraud broth at pH 4.0 and the different cellular fatty acid composition clearly distinguished PR24T from the reference strain of P. blaschkeae. The combination of genotypic and phenotypic data indicates that strain PR24T represents a subspecies of P. blaschkeae, for which the name Prototheca blaschkeae subsp. brasiliensis subsp. nov. is proposed. The respective type strain is PR24T (=DSM 103592T=IHEM 26958T).
metadata.artigo.dc.language: en_US
Appears in Collections:DMV - Artigos publicados em periódicos

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