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dc.creatorSoares, Maria A. A.-
dc.creatorCosta, José J. N.-
dc.creatorVasconcelos, Gisvani L.-
dc.creatorRibeiro, Regislane P.-
dc.creatorSouza, José C.-
dc.creatorSilva, André L. C.-
dc.creatorVan den Hurk, Robert-
dc.creatorSilva, José R. V.-
dc.date.accessioned2018-11-05T18:02:45Z-
dc.date.available2018-11-05T18:02:45Z-
dc.date.issued2018-
dc.identifier.citationSOARES, M. A. A. et al. Effects of frutalin on early follicle morphology, ultrastructure and gene expression in cultured goat ovarian cortical tissue. Histology and Histopathology, Murcia, v. 33, n. 1, p. 41-53, 2018.pt_BR
dc.identifier.urihttp://www.hh.um.es/Abstracts/Vol_33/33_1/33_1_41.htmpt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/31628-
dc.description.abstractFrutalin is a galactose-binding lectin that has an irreversible cytotoxic effect on HeLa cervical cancer cells, by inducing apoptosis and inhibiting cell proliferation. It was previously shown that after in vitro incubation, frutalin is internalized into HeLa cells nucleus, which indicates that frutalin apoptosis-inducing activity might be linked with its nuclear localization. Considering that drugs commonly used for cancer treatment have a deleterious effect on germ cells, the aim of this study was to evaluate the effect of frutalin on the activation, survival, ultrastructure and gene expression in follicles cultured within ovarian tissue. Goat ovarian fragments were cultured for 6 days in α-MEM⁺ alone or supplemented with frutalin (1, 10, 50, 100 or 200 µg/ml). Non-culturad and cultured tissues were processed for histological and ultrastructural analysis and they were also stored to evaluate the expression of anti- and pro-apoptotic genes by quantitative polymerase chain reaction (qPCR). The results showed that the frutalin, at all concentrations tested, reduced follicular survival when compared with control medium. Higher concentrations of frutalin (50, 100 or 200 µg/ml) also reduced follicular survival when compared with those tissues cultured with 1 or 10 µg/ml of frutalin. The ultrastructural analysis showed that atretic cultured follicles had retracted oocytes and a large number of vacuoles spread throughout the cytoplasm. In addition, signs of damage of mitochondrial membranes and cristae were observed. Moreover, although a dose-response effect on gene expression has not been observed, when compared with tissues culture in control medium, the presence of frutalin increased in mRNA expression pro-apoptotic genes. In conclusion, frutalin reduces follicular survival at all concentrations tested, its effects being more pronounced when high concentrations of this lectin (50, 100 and 200 µg/ml) are used. Gene expression profile and ultrastrutural features of cultured follicles suggest that follicular death in goat ovarian tissue cultured in presence of frutalin occurs via necrosis.pt_BR
dc.languageen_USpt_BR
dc.publisherUniversity of Murciapt_BR
dc.rightsrestrictAccesspt_BR
dc.sourceHistology and Histopathologypt_BR
dc.subjectFrutalinpt_BR
dc.subjectOvarian tissuept_BR
dc.subjectFrutalinapt_BR
dc.subjectTecido ovarianopt_BR
dc.titleEffects of frutalin on early follicle morphology, ultrastructure and gene expression in cultured goat ovarian cortical tissuept_BR
dc.typeArtigopt_BR
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