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metadata.artigo.dc.title: Optimization of artificial propagation in piracanjuba fish Brycon orbignyanus using cryopreserved semen
metadata.artigo.dc.creator: Felizardo, Viviane de Oliveira
Melo, Carlos Cicinato Vieira
Murgas, Luis David Solis
Andrade, Estefânia de Souza
Navarro, Rodrigo Diana
Freitas, Rilke Tadeu Fonseca de
metadata.artigo.dc.subject: Fish gametes
Cryopreserved semen
Fish - Artificial reproduction
Gametas de peixe
Sêmen criopreservado
Peixe - Reprodução artificial
metadata.artigo.dc.publisher: CryoLetters 2016
metadata.artigo.dc.identifier.citation: FELIZARDO, V. de O. et al. Optimization of artificial propagation in piracanjuba fish Brycon orbignyanus using cryopreserved semen. CryoLetters, [S. l.], v. 37, n. 5, p. 330-334, 2016.
metadata.artigo.dc.description.abstract: BACKGROUND: Cryopreserved semen could facilitate procedures during the artificial reproduction in fish. Factors affecting cryopreservation efficiency are important to define efficient protocols. OBJECTIVE: This study investigated the application of cryoprotectants on the quality of piracanjuba fish semen, the sperm concentration required for oocyte fertilization and spermatic activation. MATERIALS AND METHODS: We evaluated two intracellular cryoprotectant solutions (DMSO and methanol) and two extracellular cryoprotectant solutions (egg yolk and lactose) to cryopreserved piracanjuba semen. Sperm motility rate, motility duration and spermatic alterations were assessed. CONCLUSION: The protocol for piracanjuba semen cryopreservation can use solutions including either DMSO or methanol as intracellular cryoprotectant and egg yolk or lactose as extracellular cryoprotectants.
metadata.artigo.dc.language: en_US
Appears in Collections:DMV - Artigos publicados em periódicos

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