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dc.creatorFelizardo, Viviane de Oliveira-
dc.creatorMelo, Carlos Cicinato Vieira-
dc.creatorMurgas, Luis David Solis-
dc.creatorAndrade, Estefânia de Souza-
dc.creatorNavarro, Rodrigo Diana-
dc.creatorFreitas, Rilke Tadeu Fonseca de-
dc.identifier.citationFELIZARDO, V. de O. et al. Optimization of artificial propagation in piracanjuba fish Brycon orbignyanus using cryopreserved semen. CryoLetters, [S. l.], v. 37, n. 5, p. 330-334, 2016.pt_BR
dc.description.abstractBACKGROUND: Cryopreserved semen could facilitate procedures during the artificial reproduction in fish. Factors affecting cryopreservation efficiency are important to define efficient protocols. OBJECTIVE: This study investigated the application of cryoprotectants on the quality of piracanjuba fish semen, the sperm concentration required for oocyte fertilization and spermatic activation. MATERIALS AND METHODS: We evaluated two intracellular cryoprotectant solutions (DMSO and methanol) and two extracellular cryoprotectant solutions (egg yolk and lactose) to cryopreserved piracanjuba semen. Sperm motility rate, motility duration and spermatic alterations were assessed. CONCLUSION: The protocol for piracanjuba semen cryopreservation can use solutions including either DMSO or methanol as intracellular cryoprotectant and egg yolk or lactose as extracellular cryoprotectants.pt_BR
dc.subjectFish gametespt_BR
dc.subjectCryopreserved semenpt_BR
dc.subjectFish - Artificial reproductionpt_BR
dc.subjectGametas de peixept_BR
dc.subjectSêmen criopreservadopt_BR
dc.subjectPeixe - Reprodução artificialpt_BR
dc.titleOptimization of artificial propagation in piracanjuba fish Brycon orbignyanus using cryopreserved semenpt_BR
Appears in Collections:DMV - Artigos publicados em periódicos

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