Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/32819
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Campo DCValorIdioma
dc.creatorMaciel, Anna Lygia de Rezende-
dc.creatorRodrigues, Filipe Almendagna-
dc.creatorPasqual, Moacir-
dc.creatorCarvalho, Carlos Henrique Siqueira de-
dc.date.accessioned2019-02-15T14:32:11Z-
dc.date.available2019-02-15T14:32:11Z-
dc.date.issued2016-04-
dc.identifier.citationMACIEL, A. L. de R. et al. Large-scale, high-efficiency production of coffee somatic embryos. Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 16, n. 2, p. 102-107, Apr./June 2017.pt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/32819-
dc.description.abstractThe study aims to compare the efficiency of previously used liquid media for coffee, evaluating NAA concentrations in liquid medium and proline in semi-solid medium in the regeneration of somatic embryos and asses concentrations of BA and IAA in the maturation of embryos in temporary immersion bioreactors. For the regeneration of globular embryos from embryogenic aggregates and calli, we tested five concentrations (0.00, 0.25, 0.50, 1.00 and 2.00 mg L-1) of NAA in liquid medium and five concentrations (0.0, 0.5, 1.0, 2.0 and 4.0 g L-1) of proline in semisolid medium. The multiplication of embryogenic aggregates was highest in culture medium MM, reaching a density 7.5 times greater than that of the initial density. NAA promoted a linear increase in embryo regeneration. The medium containing 2.0 mg L-1 BA and 0.0 mg L-1 IAA yielded the highest percentage of large cotyledonary embryos.pt_BR
dc.languageen_USpt_BR
dc.publisherCrop Breeding and Applied Biotechnologypt_BR
dc.rightsacesso abertopt_BR
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.sourceCrop Breeding and Applied Biotechnologypt_BR
dc.subjectVegetative propagationpt_BR
dc.subjectBiotechnologypt_BR
dc.subjectTissue culturept_BR
dc.subjectCotyledonary embryospt_BR
dc.subjectPropagação vegetativapt_BR
dc.subjectBiotecnologiapt_BR
dc.subjectCultura de tecidospt_BR
dc.subjectEmbriões cotiledonarespt_BR
dc.titleLarge-scale, high-efficiency production of coffee somatic embryospt_BR
dc.typeArtigopt_BR
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