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dc.creatorLeite, Hianna Almeida Câmara-
dc.creatorSilva, Anderson Barbosa-
dc.creatorGomes, Fábio Pinto-
dc.creatorGramacho, Karina Peres-
dc.creatorFaria, José Cláudio-
dc.creatorSouza, Jorge Teodoro de-
dc.creatorLoguercio, Leandro Lopes-
dc.date.accessioned2019-12-12T16:41:41Z-
dc.date.available2019-12-12T16:41:41Z-
dc.date.issued2013-
dc.identifier.citationLEITE, H. A. C. et al. Bacillus subtilis and Enterobacter cloacae endophytes from healthy Theobroma cacao L. trees can systemically colonize seedlings and promote growth. Applied Microbiology and Biotechnology, [S.l.], v. 97, p. 2639-2651, 2013.pt_BR
dc.identifier.urihttps://link.springer.com/article/10.1007/s00253-012-4574-2pt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/38198-
dc.description.abstractClonal genotypes resistant to fungal diseases are an important component of the cocoa production system in southeastern Bahia state (Brazil), so that technologies for faster production of stronger and healthier plantlets are highly desirable. In this study, the effects of inoculated bacterial endophytes isolated from healthy adult cacao plants on seedlings, and aspects related to inoculation methods, colonization patterns, and photosynthesis were investigated. Sequencing of 16S rRNA, hsp-60, and rpo-B genes placed the wild-type isolates within the species Enterobacter cloacae (isolates 341 and 344) and Bacillus subtilis (isolate 629). Spontaneous rifampicin-resistant (rifR) variants for 344 were also produced and tested. Endophytic application was either by immersion of surface sterilized seeds in bacterial suspensions or direct inoculation into soil, 20 days after planting non-inoculated seeds into pots. Results from in vitro recovery of inoculated isolates showed that the wild-type endophytes and rifR variants systemically colonized the entire cacao seedlings in 15–20 days, regardless of the inoculation method. Some endophytic treatments showed significant increases in seedlings’ height, number of leaves, and dry matter. Inoculation methods affected the combined application of endophytes, which maintained the growth-promotion effects, but not in the same manner as in single applications. Interestingly, the 344-3.2 rifR variant showed improved performance in relation to both the wild type and another related variant. Photosynthetic rates and stomatal conductance increased significantly for some endophytic treatments, being partially associated with effects on growth and affected by the inoculation method. The results suggest that E. cloacae and B. subtilis endophytes from healthy adult plants (not transmitted by seeds) were able to promote vegetative growth on cacao seedlings. The development of products for large-scale use in seedlings/plantlets production systems was discussed.pt_BR
dc.languageen_USpt_BR
dc.publisherSpringerpt_BR
dc.rightsrestrictAccesspt_BR
dc.sourceApplied Microbiology and Biotechnologypt_BR
dc.subjectEndophytic colonizationpt_BR
dc.subjectHologenome theorypt_BR
dc.subjectHolobiontpt_BR
dc.subjectEnterobacteriaceaept_BR
dc.subjectNurseries productionpt_BR
dc.subjectPlant growthpromoting (rhizo)bacteria (PGPR)pt_BR
dc.subjectSeedlingspt_BR
dc.subjectSpore formingpt_BR
dc.titleBacillus subtilis and Enterobacter cloacae endophytes from healthy Theobroma cacao L. trees can systemically colonize seedlings and promote growthpt_BR
dc.typeArtigopt_BR
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