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Título : A morphological classification proposal for curimba (Prochilodus lineatus) sperm damages after cryopreservation
Autor: Miliorini, Aléssio Batista
Murgas, Luis David Solis
Rosa, Priscila Vieira e
Oberlender, Guilherme
Pereira, Gilmara Junqueira Machado
Costa, Diego Vicente da
Palavras-chave: Fish - Reproduction
Prochilodus lineatus
Dimethyl sulphoxide
Publicador: Wiley Online Library - John Wiley & Sons
Data da publicação: Jan-2011
Referência: MILIORINI, A. B. et al. A morphological classification proposal for curimba (Prochilodus lineatus) sperm damages after cryopreservation. Aquaculture Research, [S. I.], v. 42, n. 2, p. 177-187, Jan. 2011.
Abstract: The present study evaluated some cryoprotectants and concentrations for curimba (Prochilodus lineatus) semen preservation from a simple methodology of analysis of sperm morphology. Semen of nine males was diluted (1:4) into eight cryosolutions, all of them containing 5% of BTS™ Minitüb, still comprising methanol or dimethyl sulphoxide (DMSO) at four concentrations: 5%, 7.5%, 10% and 12.5%. Morphological sperm analysis was performed on an optical microscope at × 1000 magnification. Damages on the head, midpiece and tail rest (the main and terminal portions) were investigated and classified as primary or secondary, according to the assumed influence on fertilization. There was a linear reduction in the total damages with an increase in the cryoprotectant concentration. Samples with DMSO showed the lowest percentage of fractured tails and tail stumps. The cryopreservation protocol retained the fertilizing potential of the sperm after freezing and both methanol 5% and DMSO 7.5% conferred adequate protection to the curimba sperm cells. Sperm morphology, as pointed out here, must be incorporated into routine fish sperm (cryopreserved or not) analysis once sperm defects influence fertilization directly. To the best of our knowledge, this was the first report on curimba sperm damages after cryopreservation by optical microscopy. The classification model presented in this study can be adequate to tackle fish sperm damages.
Idioma: en
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