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dc.creatorLelis, Flávia M. Vieira-
dc.creatorCzajkowski, Robert-
dc.creatorSouza, Ricardo Magela de-
dc.creatorRibeiro, Daniel H.-
dc.creatorVan der Wolf, Jan M.-
dc.date.accessioned2020-07-15T17:58:23Z-
dc.date.available2020-07-15T17:58:23Z-
dc.date.issued2014-
dc.identifier.citationLELIS, F. M. V. et al. Studies on the colonization of axenically grown tomato plants by a GFP-tagged strain of Clavibacter michiganensis subsp. Michiganensis. European Journal of Plant Pathology, Dordrecht, v. 139, p. 53-66, 2014.pt_BR
dc.identifier.urihttps://link.springer.com/article/10.1007/s10658-013-0362-9pt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/41994-
dc.description.abstractIn this study, colonization and disease development of axenically-grown tomato plants by Clavibacter michiganensis subsp. michiganensis (Cmm), the causative agent of bacterial wilt and canker, was investigated. For this, a spontaneous rifampicin resistant strain of Cmm was tagged with a marker that expressed a green fluorescent protein (GFP) in a stable way and which possessed a similar virulence to the parental strain. In vitro plants were drop-inoculated at the stem base and the population dynamics was determined by dilution pour-plating in a selective medium. At 3 h after inoculation, Cmm was already present in low densities in roots, stems and leaves. At 16 dpi, Cmm was found throughout the entire plant in high densities of ca. 1010 cfu g−1. Symptoms developed in the in vitro plants typical for Cmm, such as canker, wilting and growth reduction. The presence of Cmm in vascular and parenchymatic tissue of in vitro tomato plants was confirmed by epifluorescence stereo- and confocal laser scanning microscopy. This study showed that in vitro tomato plants can be effectively used for detailed studies on interactions between Cmm and its host, in particular if a GFP-tagged strain of the pathogen is used.pt_BR
dc.languageen_USpt_BR
dc.publisherEuropean Foundation for Plant Pathologypt_BR
dc.rightsrestrictAccesspt_BR
dc.sourceEuropean Journal of Plant Pathologypt_BR
dc.subjectBacterial cankerpt_BR
dc.subjectGreen fluorescent proteinpt_BR
dc.subjectConfocal laser scanning microscopypt_BR
dc.subjectEpifluorescence stereomicroscopypt_BR
dc.subjectCâncer bacterianopt_BR
dc.subjectProteína verde fluorescentept_BR
dc.subjectMicroscopia confocal de varredura a laserpt_BR
dc.subjectEstereomicroscopia de epifluorescênciapt_BR
dc.titleStudies on the colonization of axenically grown tomato plants by a GFP-tagged strain of Clavibacter michiganensis subsp. michiganensispt_BR
dc.typeArtigopt_BR
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