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Campo DCValorIdioma
dc.creatorOliveira, V. M.-
dc.creatorMansanares, M. E.-
dc.creatorSemir, J.-
dc.creatorForni-Martins, E. R.-
dc.date.accessioned2020-12-02T21:09:25Z-
dc.date.available2020-12-02T21:09:25Z-
dc.date.issued2012-12-
dc.identifier.citationOLIVEIRA, V. M. et al. Karyotype characterization of two populations of Vernonia geminata (Asteraceae, Vernonieae) using banding and FISH techniques. Genetics and Molecular Research, Ribeirão Preto, v. 11, n. 4, p. 4204-4212, Dec. 2012. DOI: 10.4238/2012.September.25.1.pt_BR
dc.identifier.urihttps://www.geneticsmr.com/articles/1879pt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/45728-
dc.description.abstractIn order to extend our knowledge concerning karyotypes of the genus Vernonia, we applied various techniques of chromosome banding, including AgNOR and triple staining with the fluorochromes CMA/DA/DAPI (CDD), and of fluorescent in situ hybridization (FISH) for the 45S rDNA probe to specimens of two populations of Vernonia geminata collected from an open-pasture area, in southern Brazil. B chromosomes were observed in one of the populations. Both populations of V. geminata presented a pair of CMA3+ terminal bands and one pair of chromosomes with terminal AgNOR banding. The FISH evidenced, in one population, two pairs of small sites of 45S rDNA; these being two small terminal sites and two centromeric sites. In the other population, there was only one pair of small terminal sites and two sites in two B chromosomes, one in each chromosome. There was coincidence of localization between CMA+ and NOR bands with one of the small terminal sites of 45S rDNA of one chromosome of the normal complement, but not in B chromosomes. In order to extend our knowledge concerning karyotypes of the genus Vernonia, we applied various techniques of chromosome banding, including AgNOR and triple staining with the fluorochromes CMA/DA/DAPI (CDD), and of fluorescent in situ hybridization (FISH) for the 45S rDNA probe to specimens of two populations of Vernonia geminata collected from an open-pasture area, in southern Brazil. B chromosomes were observed in one of the populations. Both populations of V. geminata presented a pair of CMA3+ terminal bands and one pair of chromosomes with terminal AgNOR banding. The FISH evidenced, in one population, two pairs of small sites of 45S rDNA; these being two small terminal sites and two centromeric sites. In the other population, there was only one pair of small terminal sites and two sites in two B chromosomes, one in each chromosome. There was coincidence of localization between CMA+ and NOR bands with one of the small terminal sites of 45S rDNA of one chromosome of the normal complement, but not in B chromosomes.pt_BR
dc.languageenpt_BR
dc.publisherFundação de Pesquisas Científicas de Ribeirão Preto – FUNPEC-RPpt_BR
dc.rightsrestrictAccesspt_BR
dc.sourceGenetics and Molecular Researchpt_BR
dc.subjectAgNOR bandingpt_BR
dc.subjectCMA bandingpt_BR
dc.subjectPhysical mappingpt_BR
dc.subject45S rDNA sitept_BR
dc.titleKaryotype characterization of two populations of Vernonia geminata (Asteraceae, Vernonieae) using banding and FISH techniquespt_BR
dc.typeArtigopt_BR
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