Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/50542
Título: Curva de crescimento de calos de Enterolobium contortisiliquum induzidos in vitro
Título(s) alternativo(s): Calli growth curve of Enterolobium contortisiliquum in vitro induced
Palavras-chave: Plant tissue culture
Growth regulators
Secondary metabolites
Enterolobium contortisiliquum
Plant tissue culture
Growth regulators
Secondary metabolites
Data do documento: 2022
Editor: CDRR Editors
Citação: RODRIGUES, F. A. et al. Curva de crescimento de calos de Enterolobium contortisiliquum induzidos in vitro. Research, Society and Development, [S. l.], v. 11, n. 1, e24911124550, 2022. DOI: 10.33448/rsd-v11i1.24550.
Resumo: The objective of this work was to determine in vitro the growth curve of Enterolobium contortisiliquum calli. Calli from E. contortisiliquum cotyledons cultivated in MS medium, supplemented with 0.5 mg L-1 2,4-D + 2.0 mg L-1 picloram + 0.5 mg L-1 kinetin + 2.0 mg L-1 BAP and repeated twice were used., at intervals of 30 days. After the second subculture, the calli growth curve was established by determining the fresh mass (mg) from the day of inoculation (time 0). The experimental design was completely randomized with 17 treatments, constituted by the evaluation intervals (0, 7, 14, 21, 28, 35, 42, 49, 56, 63, 70, 77, 84, 91, 98, 105 and 112 days), and 24 repetitions. E. contortisiliquum presented five phases of callus development: exponential, linear, deceleration, stationary and decay. The calli growth curve for the species E. contortisiliquum started with the exponential phase and remained in this phase from days 0 to 7. The linear growth period in which the cell division rate decreased and the cell area increased was observed between the days 7 and 14. While the deceleration phase occurred from 14 to 28 days. It can be concluded that the growth curve of E. contortisiliquum calli is a sigmoid with five phases and at the end of the deceleration it is time to carry out the calli subculture, on average at 28 days of in vitro culture.
URI: https://doi.org/10.33448/rsd-v11i1.24550
http://repositorio.ufla.br/jspui/handle/1/50542
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