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dc.creatorBoiça Júnior, Arlindo Leal-
dc.creatorEduardo, Wellington Ivo-
dc.creatorSouza, Bruno Henrique Sardinha de-
dc.creatorMoraes, Renato Franco Oliveira de-
dc.creatorLouvandini, Helder-
dc.creatorBarbosa, José Carlos-
dc.creatorStout, Michael Joseph-
dc.identifier.citationBOIÇA JÚNIOR, A. L. et al. Protocol for assessing soybean antibiosis to Chloridea virescens. Entomologia Experimentalis et Applicata, Dordrecht, v. 170, n. 8, p. 689-699, Aug. 2022. DOI:
dc.description.abstractThere are no standard protocols for assessing antibiosis in soybean, Glycine max (L.) (Fabaceae), cultivars to lepidopterans, including tobacco budworm, Chloridea virescens (Fabricius) (Lepidoptera: Noctuidae), an important species that causes damage to soybean crops in several production regions. This study was conducted to assess methodologies to determine the optimal combination of larval density, confinement container size, and plant structure in soybean antibiosis assays to C. virescens. In addition, primary and secondary metabolites in plant structures were analyzed to elucidate potential chemical resistance mechanisms against this lepidopteran. Antibiosis assays to C. virescens in resistant (IAC-100) and susceptible (BR-16) soybean cultivars were performed using three larval densities (one, two, and three larvae), two confinement container sizes (150 and 300 ml), and combinations of soybean plant structures (leaves, uncut pods, leaves + uncut pods, cut pods, and leaves + cut pods). Phenol, tannin, fiber, and lignin contents were quantified in the leaves, uncut pods, and grains of the soybean cultivars and related to biological development of C. virescens. Resistance levels in soybean cultivars in antibiosis assays were best differentiated using one larva per 150-ml confinement container, fed only on soybean leaves. Higher contents of condensed tannins in the leaves of IAC-100 cultivar may be one of the main chemical mechanisms of resistance to C. virescens. Our study is the first to determine the optimal combination of larval density, confinement container size, and plant structure for antibiosis assays in soybean cultivars to C. virescens. The developed protocol will benefit high-throughput phenotyping in genetic breeding programs to obtain cultivars resistant to lepidopterans for use in integrated pest management.pt_BR
dc.publisherNetherlands Entomological Society / John Wiley & Sons, Inc.pt_BR
dc.sourceEntomologia Experimentalis et Applicatapt_BR
dc.subjectGlycine maxpt_BR
dc.subjectHigh-throughput phenotypingpt_BR
dc.subjectPhenolic compoundspt_BR
dc.subjectPlant resistancept_BR
dc.subjectResistance testspt_BR
dc.subjectTobacco budwormpt_BR
dc.subjectSoja - Doenças e pragaspt_BR
dc.subjectManejo integrado de pragaspt_BR
dc.subjectFenotipagem de alto rendimentopt_BR
dc.subjectCompostos fenólicospt_BR
dc.subjectResistência das plantaspt_BR
dc.titleProtocol for assessing soybean antibiosis to Chloridea virescenspt_BR
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