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dc.creatorResende, Carla Oliveira-
dc.creatorBetarelli, Rafael Pedroso-
dc.creatorRabelo, Stenia Severo-
dc.creatorChaves, Bruna Resende-
dc.creatorRodriguez-Gil, Joan Enric-
dc.creatorZangeronimo, Marcio Gilberto-
dc.date.accessioned2020-05-15T18:02:21Z-
dc.date.accessioned2023-06-27T18:54:03Z-
dc.date.available2020-05-15T18:02:21Z-
dc.date.available2023-06-27T18:54:03Z-
dc.date.issued2019-09-
dc.identifier.citationRESENDE, C. O. et al. Addition of insulin-like growth factor I (IGF-I) and reduced glutathione (GSH) to cryopreserved boar sêmen. Animal Reproduction Science, [S.I.], v. 208, Sept. 2019. Não paginado.pt_BR
dc.identifier.urihttps://www.sciencedirect.com/science/article/abs/pii/S037843201930185X?via%3Dihub#!pt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/57459-
dc.description.abstractThe objective of this study was to evaluate the effect of adding reduced glutathione (GSH) to a boar semen freezing extender supplemented with insulin-like growth factor I (IGF-I) or anti-IGF-I. Eight ejaculates from eight boars were extended to obtain insemination doses, which were supplemented with either recombinant human IGF-I (30 ng/mL) or anti-IGF-I (60 ng/mL) shortly after extension. After 24 h of liquid storage at 17 °C, the semen was frozen with or without GSH (5 mM) in the freezing extender for a total of six treatments. Osmotic resistance and acrosome integrity was greater in fresh semen (P <  0.05) soon after adding IGF-I or the anti-IGF-I antibody. After 24 h of cooling, the supplementation with these compounds resulted in an increased (P <  0.05) percentage of sperm with relatively greater mitochondrial activity and reduced the percentage of cells with relatively greater concentrations of superoxide. After thawing, there was a reduction (P <  0.05) in the percentage and fluorescence intensity of sperm with greater quantities of superoxide and peroxide only in samples treated with GSH + IGF-I and GSH + anti-IGF-I. The addition of GSH (alone or in combination with IGF-I or anti-IGF-I), however, reduced the percentage of sperm with an intact acrosome (P < 0.05). The same effect was not observed with IGF-I or anti-IGF-I alone. In conclusion, the addition of IGF-I or anti-IGF-I improved the quality of fresh or liquid-stored semen. Using GSH in the freezing extender improved the antioxidant potential of frozen semen only in combination with IGF-I or an anti-IGF-I antibody.pt_BR
dc.languageenpt_BR
dc.publisherElsevier B.V.pt_BR
dc.rightsrestrictAccesspt_BR
dc.sourceAnimal Reproduction Sciencept_BR
dc.subjectAntioxidantpt_BR
dc.subjectBoar spermatozoapt_BR
dc.subjectCryopreservationpt_BR
dc.subjectLipid peroxidationpt_BR
dc.subjectSemenpt_BR
dc.subjectThawed semenpt_BR
dc.subjectAntioxidantept_BR
dc.subjectSuíno - Espermatozóidespt_BR
dc.subjectCriopreservaçãopt_BR
dc.subjectPeroxidação lipídicapt_BR
dc.subjectSêmenpt_BR
dc.subjectSêmen descongeladopt_BR
dc.titleAddition of insulin-like growth factor I (IGF-I) and reduced glutathione (GSH) to cryopreserved boar semenpt_BR
dc.typeArtigopt_BR
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