Análise da relação entre umidade e atividade biológica em sementes de feijão (phaseolus vulgaris l.), através do método biospeckle e do algoritmo momento de inércia

Abstract

A new technique for analysis of the seeds viability and vigor is based on the phenomenon known as biospeckle or dynamic speckle, that is resulted of the biological materials illumination with laser light forming an interference figure that reminds a boiling liquid. When a material presents great activity, the image of generated speckle shows a very dynamic changes, while materials with low activity shows an interference pattern that modifies more softly, and the evaporation of the water can mask the speckle information. The goal of this work is, then, to contribute for the development of this methodology, and to determine a limit humidity level in that is possible to use with safety this technique. To reach such purposes, took place the analysis of the relationship between humidity and values of biological activity gotten through the biospeckle of bean seeds. The Moment of Inertia, that is an image-processing algorithm, was used to quantify the speckle pattern. It was concluded that the maximum limit of work with this method is 19% b.u. After this value, the information returned by the dynamic speckle become strongly affected by the humidity and, consequently, inconsistent.