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Autocompaixão e fatores associados ao risco para transtornos alimentares: um estudo comparativo entre estudantes e profissionais de nutrição
(Universidade Federal de Lavras, 2025-08-21) Mangia, Maykeline Stéphani Pereira; Teixeira, Lílian Gonçalves; Souza, Carolina Belomo de; Consolli, Marcella Lobato Dias; Soares, Fabíola Lacerda Pires
Eating behavior involves multiple and complex actions, since the act of eating involves conditions beyond physical needs, being also influenced by emotional, cognitive, and social factors. Within this broad context of eating behavior and its current changes, nutrition students and professionals appear as groups susceptible to developing eating disorder risk or the disorder itself, given social pressure, stress, anxiety, and depression. In this scenario, self-compassion seems to be associated with positive mental health outcomes, acting as a protective factor against disordered eating behaviors. Objective: To compare the risk of eating disorders between nutrition students and professionals and to evaluate the determinants related to nutrional status, mental health, and self-compassion. Methodology: A cross-sectional study was conducted between March and June 2024, using a self-administered online questionnaire containing sociodemographic data, the Eating Attitudes Test (EAT-26), the Depression, Anxiety and Stress Scale (DASS-21), and the Self-Compassion Scale (SCS). Results: A total of 419 women participated, including 253 nutrition students and 166 nutrition professionals. A higher prevalence of overweight and obesity was found among professionals compared to students, while the latter showed a higher percentage of underweight. The mean total EAT-26 scores suggested that both students and professionals had a low prevalence of developing eating disorders (EAT-26 total < 20). However, approximately one-third of the sample showed a high risk for these disorders. Students presented significantly higher total EAT-26 scores compared to professionals. For both groups, an increase in body mass index (BMI) was significantly associated with a higher risk of eating disorders (EAT-26 > 20). Among students, a higher risk was also associated with increased anxiety. Among nutrition professionals, higher levels of isolation and self-kindness were associated with a lower risk of eating disorders. Interestingly, higher scores in the common humanity subscale were associated with greater risk. Conclusion: Nutrition students and professionals show a low prevalence of eating disorders; however, one-third of the sample presents an elevated risk. Higher levels of anxiety were a significant predictor of risk among students. Among professionals, self-kindness and isolation acted as protective factors, while higher scores on the common humanity subscale indicated increased risk. Overall, self-compassion may represent a relevant preventive strategy with positive impacts on psychological and behavioral aspects related to professional training, mental health, and quality of life.
Temporal transcriptomic profiling of Colletotrichum lindemuthianum race 65 during compatible and incompatible interaction with common bean
(Universidade Federal de Lavras, 2025-10-30) Rodrigues, Laís Nóbrega; Pereira, Welison Andrade; Novaes, Evandro; Pylro, Victor Satler; Vasconcellos, Renato Coelho de Castro; Costa, Larissa Carvalho
Anthracnose, caused by Colletotrichum lindemuthianum, is one of the most severe diseases affecting common bean (Phaseolus vulgaris), leading to significant yield losses in major production regions. Since this pathogen is characterized by high variability and a remarkable ability to overcome plant defense mechanisms, understanding the infection process at the molecular level is essential for developing more effective and durable control strategies. In this context, the present study aimed to investigate the molecular basis of the infection process caused by race 65 of the pathogen in contrasting bean genotypes (Ouro Vermelho, resistant, and BRS Estilo, susceptible) through a time-series analysis of the fungal transcriptome (0, 48 and 96 hpi) using RNA-seq. The identification and characterization of differentially expressed genes, combined with the analysis of functional categories essential for pathogenicity, enabled inferences about the adaptive strategies employed by the fungus during the infection process. The prediction of secreted proteins revealed the presence of six and seven CAZymes in the Inter A and Inter V groups, respectively, at 96 hpi, with a predominance of enzymes belonging to the glycosyl hydrolase (GH) class. Among the 21 transport-related proteins identified, heat shock proteins (HSPs) and MFS transporters were particularly prominent, especially within the Inter V subset (96 hpi). In addition, 15 Candidate Secreted Effector Proteins (CSEPs) were detected, three of which showed significant similarity to proteins recorded in the PHI-base database. Overall, these results provide molecular insights into the pathogenicity of C. lindemuthianum and highlight potential targets for breeding strategies aimed at achieving durable resistance to anthracnose in common bean.
Caracterização histológica da organogênese in vitro de Eucalyptus urophylla e Eucalyptus grandis
(Universidade Federal de Lavras, 2025-09-30) Oliveira, Lara Beatriz; Paiva, Luciano Vilela; Pádua, Marlúcia Souza; Esteves, Giovana; Marinho, Tácila Rayene dos Santos; Timoteo, Caroline de Oliveira
Eucalyptus, a species native to Australia, has great commercial value for the pulp and paper, coal, and energy industries, as well as for the pharmaceutical and cosmetics industries. Brazil is the largest exporter of pulp and paper, and eucalyptus is the most widely cultivated crop in Brazilian forestry, occupying 77% of the planted area. In vitro propagation and cultivation methods are used in laboratories to develop eucalyptus seedlings with the desired resistance and productivity characteristics. Among these methodologies, callogenesis and regeneration are employed as a means of obtaining such seedlings through the appropriate use of plant growth regulators. Therefore, the objective of this study was to evaluate the origin of callus shoots in Eucalyptus grandis and Eucalyptus urophylla during the regeneration stage, induced by the use of different concentrations of the plant growth regulator Thidiazuron (TDZ). A callus culture was established from leaf, cotyledon, and apex explants taken from plants grown from seeds six weeks after germination. After 30 days of callus formation, the calli were allowed to regenerate. Histological sections were taken from calli of Eucalyptus grandis and Eucalyptus urophylla, which had two months of regeneration. Eucalyptus grandis was the most responsive genotype, presenting the highest number of shoots. The most effective TDZ concentration was 0.025 mg/L for Eucalyptus grandis and 0.025 mg/L and 0.050 mg/L for Eucalyptus urophylla. The apex was the best explant type used in this experiment, as it presented better regeneration than the others. Analysis of the histological section images showed that, as the sections progressed, the origin of the shoots became visible. It is concluded that the shoots present in the callus of both eucalyptus species arose from cells close to the conduction systems of the original explant, where young vascular bundles are located close to mature ones, providing structural support and local signaling that favor regeneration. These results yielded useful information on how choices of genotype, explant type, and growth regulator concentration influence the callus formation and shoot regeneration stages in eucalyptus. Histological analyses revealed the location of the shoots' origin, crucial information for subsequent genetic transformation protocols.
Efeitos letais e subletais do isocicloseram sobre Myzus persicae e seu predador natural Allograpta exotica
(Universidade Federal de Lavras, 2025-08-07) Versanne, Romario; Haddi, Khalid; Silva, Ricardo Siqueira da; Tavares, Clebson dos Santos
Insect pests, including the green peach aphid Myzus persicae, cause significant damage to various crops, and the use of insecticides is a common method for their control. Hoverfly larvae are natural predators of aphids and other plant-sucking insects and play a critical role in regulating pest populations in agricultural systems. However, the use of insecticides can have lethal and sublethal effects on these beneficial insects, potentially affecting their biological traits and their effectiveness in aphid control. This study aimed to evaluate the lethal and sublethal effects of isocycloseram on the longevity and reproductive traits of M. persicae and its natural predator Allograpta exotica, focusing on the following biological traits such as longevity, fecundity, pupal weight, developmental time, and predation rate. The study was carried out at the Molecular Entomology and Ecotoxicology Laboratory (MEET) at the Federal University of Lavras. The rearing of M. persicae was maintained under controlled conditions at 20 ± 2 °C, 70 ± 10% relative humidity, and a 16-hour photoperiod, whereas A. exotica was kept in a climatic chamber at 25 ± 2 °C, 50 ± 7% relative humidity, and photoperiod of 12:12 h. Toxicological bioassays consisted of exposing adult M. persicae and A. exotica larvae to seven concentrations to determine the LC 50 and LC 90 values. Mortality caused by isocycloseram was assessed after 48 hours, and the dose-response curve was determined. Subsequently, the sublethal effects on adult M. persicae and A. exotica larvae were evaluated. Fifty repetitions for 5 replicates of adults M. persicae and fifty repetitions for 3 replicates of second instar larvae of A. exotica were exposed to sublethal concentrations (LC 1 , LC 5 , LC 10 , LC 25 ) and to the control. The longevity and fecundity of M. persicae were recorded daily until death. The evaluation of A. exotica larvae began at the second instar and continued until the death of the newly emerged adults. Each larva was offered 20 adult aphids per day, to assess its development time and predatory capacity, and the biological traits of adults emerging from sublethal exposed larvae. The results showed significant differences among all evaluated parameters. However, according to our findings, the use of isocycloseram in Integrated Pest Management (IPM) programs requires further research to ensure the safety of natural enemies.