Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/11188
Title: Estabilidade da fitase e sua utilização na alimentação de frangos de corte
Other Titles: Stability of phytase and its utilization in the feeding of broiler chickens
Authors: Teixeira, Antônio Soares
Bertechini, Antônio Gilberto
Santos, Custódio Donizete dos
Garcia, Edivaldo Antônio
Freitas, Rilke Tadeu Fonseca de
Keywords: Frango de corte - Alimentação e rações
Enzimas na nutrição animal
Fitase
Broilers (Poultry) - Feeding and feeds
Enzymes in animal nutrition
Phytase
Issue Date: 24-May-2016
Publisher: Universidade Federal de Lavras
Citation: PIZZOLANTE, C. C. Estabilidade da fitase e sua utilização na alimentação de frangos de corte. 2000. 117 p. Tese (Doutorado em Zootecnia)-Universidade Federal de Lavras, Lavras, 2000.
Abstract: This work was designed to evaluate the effect of storage forms and conditions upon the enzyme activity of phytase and bioavaibility of calcium and phosphorus in broiler diets. The work was accomplished in two steps. The first step, made in the laboratory measured the activity of the phytase enzyme along the storage period. In this step, two experiments were performed: Experiment 1, constituted of 5 treatments (pure phytase stored at 0 °C, 4 °C and environmental temperature and mixed to vitamin and mineral supplement, stored at environmental temperature) in CRD and split plot scheme. The activities were evaluated every 14 days for 112 days of storage, being verified that the phytase storage in the pure form at 0o C was superior to the other treatments. Experiment 2, made up of 4 treatments (phytase mixed to the ration directly, directly and afterwards pelleted, via mineral supplement and via vitamin supplement),all the treatments being stored at environmental temperature, in CRD and split plot scheme. The activities were evaluated every 7 days for 56 days' storage, being verified that the storage of the phytase mixed to the ration via vitamin supplement and directly with the ration pelleted later, provoked a fall in phytase activity when compared with the other treatments. In the second step, the effect of phytase on the bioavaibility of calcium and phytic phosphorus was evaluated, 2 experiments being accomplished (3 and 4); in both experiments were utilized 576 broiler line chicks, housed in an array of heated batteries, receiving practical diets on the basis of corn and soybean meal (basal) for 21days. At the end of 27 days of age,96 birds were slaughtered for evaluation of the mineral contents (Ca and P) in the tíbias and plasma phosphorus. The excretae were collected from 22 to 27 days of age of the birds. Experiment 3: A CRD with the treatments in 2 x 3 x 2 +4 factorial arrangement was utilized, namely, two levels of total phosphorus (0.35 and 0.45% of total phosphorus), three leveis of phytase (500, 750 and 1,000 FTU) and four additional treatments with levels of 0.35 and 0.45 % of available phosphorus for each sex, with three replicates per treatment. There was significant interaction among levels of phosphorus and phytase (P< 0.05) for weight gain, ration consumption and feed conversion. Phytase did not indicate significant differences when the level 0.45% was utilized, nevertheless, at the level 0.35% as phytase was supplemented, weight gain, ration consumption and feed conversion were improved, chiefly with 1,000 FTU/Kg, in both sexes. The males presented greater weight gain. The ration consumption and feed conversion were equal to those of females. The contrast 0.45% did not affect the performance of males and females, the same not occurring with the level 0.35%,at which the available phosphorus was superior in both the sexes. The highest contents of ashes, phosphorus and calcium in the tíbias and plasma phosphorus were obtained with the levels of 750 and 1,000 FTU/Kg of phytase and 0.45% of total phosphorus. The males presented higher contents of ashes in the tibias. The level 0.45% of available phosphorus presented the greatest contents of ashes, calcium and phosphorus in the tibias, and phosphorus in the plasma. The lowest excretions of phosphorus occurred at the levels 0.35% of total phosphorus and 1,000FTU/kg of phytase. The lowest contents of ashes and calcium in the excretae were obtained with 0.35% and 1,000FTU/Kg of phytase. The females excreted smallest amounts of ashes, calcium and phosphorus than the males. Experiment 4: a CRD with the treatments in 3 x 4 x 2 factorial arrangement, namely, three levels of phytase (0, 500 and 1,000 FTU), four levels of calcium (0.7, 0.8, 0.9 and 1.0%) with four replicates per treatment. The performance was not affected by the treatments utilized, the males being superior to the females in weight gain, feed consumption and conversion. The contents of ashes in the tibias were not affected by the levels of phytase but as calcium levels raised, the ash contents increased. The contents of calcium and phosphorus in the tibias increased with the supplementation of 500 and 1,000 FTU/kg of phytase and with calcium levels. The utilization of phytase did not decrease the excretion of ashes, calcium and phosphorus.
Description: Dissertação/tese está disponível online com base na Resolução CEPE nº 090, de 24 de março de 2015, que dispõe sobre a disponibilização da coleção retrospectiva de teses e dissertações online no Repositório Institucional da UFLA, sem autorização prévia dos autores. Parágrafo Único. Caberá ao autor ou orientador a solicitação de restrição quanto à divulgação de teses e dissertações com pedidos de patente ou qualquer embargo similar. Art. 5º A obra depositada no RIUFLA que tenha direitos autorais externos à Universidade Federal de Lavras poderá ser removida mediante solicitação por escrito, exclusivamente do autor, encaminhada à Comissão Técnica da Biblioteca Universitária. / Arquivo gerado por meio da digitalização de material impresso. Alguns caracteres podem ter sido reconhecidos erroneamente.
URI: http://repositorio.ufla.br/jspui/handle/1/11188
Appears in Collections:Zootecnia - Doutorado (Teses)

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