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Título: Genetic transformation with the gfp gene of Colletotrichum gloeosporioides isolates from coffee with blister spot
Autor(es): Armesto, Cecilia
Maia, Fernanda Gonçalves Martins
Abreu, Mário Sobral de
Figueira, Antonia dos Reis
Silva, Bruno Marques da
Monteiro, Fernando Pereira
Assunto: Colletotrichum gloeosporioides
Fluorescent proteins
Pathogenicity
Publicador: Sociedade Brasileira de Microbiologia
Data de publicação: Jul-2012
Referência: ARMESTO, C. et al. Genetic transformation with the gfp gene of Colletotrichum gloeosporioides isolates from coffee with blister spot. Brazilian Journal of Microbiology, São Paulo, v. 43, n. 3, p. 1222-1229, July 2012.
Abstract: Blister spot (Colletotrichum gloeosporioides) is now widespread in most coffee producing states of Brazil, becoming a limiting factor for production. The lack of data relating to the reproduction of typical symptoms (light green, oily patches) leaves a gap within the pathosystem, forcing the search for new methodologies for monitoring the disease. Monitoring of genetically modified organisms has proven to be an effective tool in understanding the host x pathogen interactions. Thus, the present study was carried out to evaluate the effectiveness of two systems of genetic transformation in obtaining mutants using the gfp reporter gene. Using the two transformation systems (PEG and electroporation) revealed the efficiency of both, confirmed by fluorescence microscopy and resistance to the antibiotic hygromycin-B, when incorporated into the culture medium. The fungus maintained its cultural and morphological characteristics when compared to wild strains. When inoculated on coffee seedlings, it was found that the pathogenicity of the processed isolates had not changed.
URI: http://repositorio.ufla.br/jspui/handle/1/12556
Idioma: en_US
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