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dc.creatorAndrade, Thaís de-
dc.creatorVon Pinho, Edila Vilela de Resende-
dc.creatorVon Pinho, Renzo Garcia-
dc.creatorOliveira, Gustavo Evangelista-
dc.creatorV., Andrade-
dc.creatorFernandes, Joana Souza-
dc.identifier.citationANDRADE. T. de. et al. Physiological quality and gene expression related to heat-resistant proteins at different stages of development of maize seeds. Genetics and Molecular Research, Ribeirão Preto, v. 12, n. 3, p. 3630-3642, 2013.pt_BR
dc.description.abstractWe quantified and characterized the expression of heatresistant proteins during seed development of maize lines with distinct levels of tolerance to high drying temperature. A corn field was planted for multiplication of seeds of different lines, two tolerant and two nontolerant to high drying temperatures. Harvest of the seeds was carried out at various stages of development and they were then subjected to tests of moisture content, germination, first count of germination, accelerated aging, and cold test. The seeds were stored in a freezer for later analysis of expression of heat-resistant proteins by means of real-time PCR, electrophoresis, and spectrophotometry. We observed that heat-resistant proteins are expressed in a differential manner in seeds from different lines and at different stages of development. The expression of heatresistant proteins was earlier in lines tolerant to high drying temperatures. Greater germination and vigor values was found for seeds collected at the last stage of development.pt_BR
dc.publisherFundação de Pesquisas Científicas de Ribeirão Pretopt_BR
dc.sourceGenetics and Molecular Researchpt_BR
dc.subjectZea mayspt_BR
dc.subjectLate Embryogenesis Abundant ( LEA )pt_BR
dc.titlePhysiological quality and gene expression related to heat-resistant proteins at different stages of development of maize seedspt_BR
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