Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/28512
Title: Morphological and ultrastructural analysis of various types of banana callus, cv. Prata anã
Other Titles: Análise morfológica e ultra-estrutural de diferentes calos de bananeira, cv. Prata anã
Keywords: Scanning electron microscopy
Transmission electron microscopy
Musa sp.
Cytology
Embryogenesis
Microscopia eletrônica de varredura
Microscopia eletrônica de transmissão
Citologia
Embriogênese
Issue Date: 2012
Publisher: Editora da Universidade Estadual de Maringá - EDUEM
Citation: RIBEIRO, L. de O. et al. Morphological and ultrastructural analysis of various types of banana callus, cv. Prata anã. Acta Scientiarum. Agronomy, Maringá, v. 34, n. 4, p. 423-429, Oct./Dec. 2012.
Abstract: This work was carried out to characterise morphologically and ultrastructurally the banana callus, obtained from the scalp method. Genotypes of banana, cv. Prata anã, cultivated in vitro were used to induce meristems at the leaf base; subsequently, structures known as scalps were formed. For ultrastructural analysis, five samples of callus were collected, fixed in Karnovsky solution and analysed by scanning electron microscope (SEM) and transmission electron microscope (TEM). The formation of three types of callus was observed: Type 1 - transparent watery callus, Type 2 - yellow callus with small clusters, Type 3 - yellow callus with large clusters. SEM analysis showed that Type 1 callus cells were elongated and that Type 2 and Type 3 callus cells were isodiametric, which is a characteristic of embryogenic cells. The TEM analysis showed that Type 1 callus cells had thin walls, a large number of small vacuoles and dispersed cytoplasm. The Type 2 callus cells showed dense cytoplasm, large vacuoles and a large amount of mitochondria. The Type 3 callus cells had thick and intercellular spaces. Thus, the Type 2 callus cells had characteristics consistent with embryogenic callus cells.
URI: http://repositorio.ufla.br/jspui/handle/1/28512
Appears in Collections:DFP - Artigos publicados em periódicos
DQI - Artigos publicados em periódicos



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