Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/28966
Título: Criopreservação de gemas laterais de mangabeira: o papel da prolina
Título(s) alternativo(s): Cryopreservation of mangabeira axillary: the hole of proline
Palavras-chave: Mangabeira – Germinação – Testes
Criopreservação
Sementes recalcitrantes
Mangabeira – Germination – Testing
Cryopreservation
Recalcitrant seeds
Hancornia speciosa
Data do documento: Set-2014
Editor: Universidade Federal de Campina Grande
Citação: PRUDENTE, D. de O. et al. Criopreservação de gemas laterais de mangabeira: o papel da prolina. Saúde e Ciência, Campina Grande, v. 3, n. 3, p. 86-93, set./dez. 2014.
Resumo: Mangabeira (Hancornia speciosa Gomes) fruit species native of Brazil has economic potential for exploitation of drugs or fruit production. However, the species has a low germination rate and recalcitrant seeds. In this context, the objective of this study was to test the effect of proline modify the step of pre-cultivation of lateral gems before cryopreservation. The gems were extracted from shoots grown in vitro mangabeira and pre-grown in WPM culture medium supplemented with 0.3 M sucrose combined with different concentrations of proline (0.0, 0.1, 0.2 and 0.3 M) and for different times (24 and 48 hours). After the pre-cultivation the shoots were treated with PVS2 at 0 °C for 15 minutes and cryopreserved by vitrification droplet. After cryopreservation, the samples were reheated and gems cultivated in WPM containing 0.2 μM BAP in a growth chamber with a 16h photoperiod. After 30 days of culture were evaluated survival and regeneration of shoots by Skott-Knott test (p ≤ 0.05). The preculture in 0.3 M sucrose + 0.1 M proline for 24 hours caused a significant increase in the percentage of survival (83.3%) and regeneration of shoots (93.3%).
URI: http://www.ufcg.edu.br/revistasaudeeciencia/index.php/RSC-UFCG/article/view/176
http://repositorio.ufla.br/jspui/handle/1/28966
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