Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/30408
Title: Intra- and intergenomic chromosomal pairing in artificially polyploidized elephant grass and pearl millet hybrids
Other Titles: Pareamento cromossômico intra e intergenômico em híbridos de capim-elefante e milheto poliploidizados artificialmente
Keywords: Pennisetum glaucum
Pennisetum purpureum
In situ hybridization
Intergenomic pairing
Interspecific hybridization
Hibridização in situ
Pareamento intergenômico
Hibridação interespecífica
Issue Date: Sep-2017
Publisher: Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
Secretaria de Pesquisa e Desenvolvimento (SPD)
Citation: SANTOS, F. M. da C. et al. Intra- and intergenomic chromosomal pairing in artificially polyploidized elephant grass and pearl millet hybrids. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 52, n. 9, p. 814-817, set. 2017.
Abstract: The objective of this work was to evaluate, by genomic in situ hybridization (GISH), pairing configurations as potential indicators of recombination between chromosomes of different parental genomes, in two interspecific hybrids (elephant grass x pearl millet) artificially polyploidized. Anthers from young flower buds were used in the chromosomal preparations. The genomic probe was prepared with pearl millet DNA and labeled with biotin-16-dUTP by the nick translation reaction. Blocking DNA was prepared with genomic elephant grass DNA. The homoeologous intergenomic pairing, observed in the two hybrids, indicates the possibility of recombination between chromosomes of the parental genomes.
URI: http://repositorio.ufla.br/jspui/handle/1/30408
Appears in Collections:DBI - Artigos publicados em periódicos



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