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http://repositorio.ufla.br/jspui/handle/1/31715| Título: | Preparation of an affinity cryogel column for lysozyme purification |
| Palavras-chave: | Lysozyme Cryogel Affinity chromatography Lisosomos Cromatografia de afinidade |
| Data do documento: | 2017 |
| Editor: | Taylor & Francis |
| Citação: | VERÍSSIMO, L. A. A. et al. Preparation of an affinity cryogel column for lysozyme purification. Separation Science and Technology, New York, v. 52, n. 12, p. 1973-1982, 2017. |
| Resumo: | Affinity cryogels were synthesized using tris(hydroxymethyl)aminomethane (Tris) as ligand for specific interactions with the lysozyme (LYZ). The cryogel was produced by cryo-copolymerization at −12°C. A central composite rotational design 22 was used to optimize the immobilization procedure of the Tris on the cryogel. A maximum adsorption for LYZ (149.5 mg/g) was achieved when 376 mg/mL of Tris and 3.06 mol/L of sodium borohydride were used during the Tris immobilization. Chromatographic separation of LYZ from chicken egg white was done with a purity of 92.13%. Results showed that the affinity cryogel was a potential separation medium for LYZ purification. |
| URI: | https://www.tandfonline.com/doi/abs/10.1080/01496395.2017.1318921?journalCode=lsst20 http://repositorio.ufla.br/jspui/handle/1/31715 |
| Aparece nas coleções: | DCA - Artigos publicados em periódicos |
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