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|Título :||Aspergillus species from brazilian dry beans and their toxigenic potential|
|Autor:||Santos-Ciscon, Bárbara Alves dos|
Machado, José da Cruz
Dias, Iara Eleutéria
|Data da publicação:||Mar-2019|
|Referência:||SANTOS-CISCON, B. A. dos et al. Aspergillus species from brazilian dry beans and their toxigenic potential. International Journal of Food Microbiology, [S.l.], v. 292, p. 91-100, Mar. 2019.|
|Abstract:||Aspergilli are common contaminants of food and feed and a major source of mycotoxins. In this study, 87 Aspergillus strains were isolated from beans from 14 different cities in Brazil and identified to the species level based on partial calmodulin and β-tubulin sequence data. All green spored isolates belonged to section Flavi and were identified as A. flavus (n = 39) or A. pseudocaelatus (n = 1). All black spored isolates belonged to section Nigri and were identified as A. niger (n = 24) or A. luchuensis (n = 10), while the yellow spored strains were identified as A. westerdijkiae (n = 7), A. ostianus (n = 3), A. ochraceus (n = 1) or A. wentii (n = 2). The toxigenic potential of these Aspergillus strains from beans was studied by the prospection of genes in three of the major mycotoxin clusters: aflatoxin (seven genes checked), ochratoxin A (four genes) and fumonisin (ten genes and two intergenic regions). Genes involved in the biosynthesis of aflatoxin were only detected in A. flavus isolates: 17/39 A. flavus isolates proved to contain all the aflatoxin genes tested, the others missed one or more genes. The full complement of fumonisin biosynthesis genes was identified in all A. niger isolates. Finally, no genes for ochratoxin A were detected in any of the isolates. Our work suggests that aflatoxin production by some A. flavus strains and fumonisin production by A. niger isolates form the largest mycotoxin risks in Brazilian beans.|
|Aparece nas coleções:||DFP - Artigos publicados em periódicos|
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