Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/37871
Título: Seleção assistida por marcadores de DNA em retrocruzamento visando resistência ao mofo branco em feijoeiro
Título(s) alternativo(s): Assisted selection by DNA markers in backcrossing aiming resistance to white mold in common bean
Palavras-chave: Sclerotinia sclerotiorum
Marcadores microssatélites
Similaridade genética
Microsatellite Markers
Genetic similarity
Data do documento: Out-2012
Editor: Universidade Federal Rural do Semi-Árido
Citação: ANTONIO, R. P.; SANTOS, J. B. dos; ALVES, F. C.; GONÇALVES, P. R. C.; LARA, L. A. de C. Seleção assistida por marcadores de DNA em retrocruzamento visando resistência ao mofo branco em feijoeiro. Revista Caatinga, Mossoró, v. 25, n. 4, p. 61-67, out-dez. 2012.
Resumo: The present study aimed to use DNA markers to select plants for the first and second backcross (BC) of two distinct populations of common bean, carrying the QTL for resistance to white mold and RAPD and microsatellite markers to identify the plants of the BC population more similar to their recurrent parent. For this, it was used the lines G122 (resistant –P1) as donor parent and VC3 (susceptible –P2) as recurrent parent for generation of the population F1RC1-GV evaluated for resistance to white mold by means of the SCAR Phsand genotyped with RAPD primers. In the F1RC2-EM population were used the lines Ex Rico 23 (resistant) as donor parent and M20 (susceptible) as recurrent parent evaluated for resistance to white mold, by means of RAPD primers O12.1600 and O15.1800 and genotyped with microsatellite (SSR) primers. Genetic similarity (sgij) between each BC plant and the recurrent parent was estimated using the Sorensen-Dice coefficient. The propor-tion of the SSR alleles derived from the recurrent parent was also estimated and, simultaneously, genetic simi-larity and the proportion of SSR alleles were efficient for identifying plants more similar to the recurrent par-ent. It was found that marker assisted selection (MAS) contributesto reducing the number of BC in at least a generation.
URI: http://repositorio.ufla.br/jspui/handle/1/37871
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