Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/38829
Title: Effects of Jatropha curcas oil inLactuca sativa root tip bioassays
Keywords: Chromosomal aberration
Germination rate
Phorbol esters
Root elongation
Jatropha curcas L.
Aberração cromossômica
Razão de germinação
Éster de forbol
Crescimento radicular
Issue Date: Mar-2014
Publisher: Academia Brasileira de Ciências
Citation: ANDRADE-VIEIRA, L. F.; BOTELHO, C. M.; LAVIOLA, B. G.; PALMIERI, M. J.; PRAÇA-FONTES, M. M. Effects of Jatropha curcas oil inLactuca sativa root tip bioassays. Anais da Academia Brasileira de Ciências, Rio de Janeiro, v. 86, n. 1, p. 373-382, 2014.
Abstract: Jatropha curcas L. (Euphorbiaceae) is important for biofuel production and as a feed ingredient for animal. However, the presence of phorbol esters in the oil and cake renders the seeds toxic. The toxicity ofJ. curcas oil is currently assessed by testing in animals, leading to their death. The identification of toxic and nontoxic improved varieties is important for the safe use of J. curcas seeds and byproducts to avoid their environmental toxicity. Hence, the aim of this study was to propose a short-term bioassay using a plant as a model to screen the toxicity of J. curcas oil without the need to sacrifice any animals. The toxicity of J. curcas oil was evident in germination, root elongation and chromosomal aberration tests in Lactuca sativa. It was demonstrated that J. curcas seeds contain natural compounds that exert phyto-, cyto- and genotoxic effects on lettuce, and that phorbol esters act as aneugenic agents, leading to the formation of sticky chromosomes and c-metaphase cells. In conclusion, the tests applied have shown reproducibility, which is important to verify the extent of detoxification and to determine toxic doses, thus reducing the numbers of animals that would be used for toxicity tests.
URI: http://repositorio.ufla.br/jspui/handle/1/38829
Appears in Collections:DBI - Artigos publicados em periódicos

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