Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/39004
Title: In vitro induction of hexaploid plants from triploid hybrids of Pennisetum purpureum and Pennisetum glaucum
Keywords: Chromosome doubling
Colchicine
Pennisetum hybrids
Flow cytometry
Duplicação cromossômica
Colchicina
Citometria de fluxo
Issue Date: Feb-2009
Publisher: Blackwell Verlag
Citation: CAMPOS, J. M. S. et al. In vitro induction of hexaploid plants from triploid hybrids of Pennisetum purpureum and Pennisetum glaucum. Plant Breeding, [S. l.], v. 128, n. 1, p. 101-104, Feb. 2009.
Abstract: This study describes an efficient in vitro method using colchicine‐treated triploid seedlings of Napiergrass and Pearl millet hybrids to produce hexaploid hybrids and their subsequent identification with flow cytometry. It also describes chromosome count and stomatal morphology and their use to ploidy analysis. Four‐hundred and eighty triploid seeds, representing 12 different hybrids were sterilized and transferred to MS media to induce chromosome doubling. Surviving plants were analysed by flow cytometry. From six triploid (control) and hexaploid plants, the stomata sizes and frequency were analysed. Chromosome count was performed only in the plants identified as hexaploid. Seventeen plants were identified as hexaploid by flow cytometry analysis. Further confirmation of the hexaploid condition was performed with stomatal morphology (stomatal frequency reduction and stomatal length increase) and chromosome count (2n = 6x = 42). Chromosome doubling has numerous applications in Pennisetum breeding. It can be used to restore the fertility of interspecific hybrids and to improve seed size.
URI: https://onlinelibrary.wiley.com/doi/full/10.1111/j.1439-0523.2008.01546.x
http://repositorio.ufla.br/jspui/handle/1/39004
Appears in Collections:DNU - Artigos publicados em periódicos

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