Evaluation of adhesive properties of presumptive probiotic Lactobacillus plantarum strains

Abstract

Thirty-two strains of Lactobacillus plantarum UFLA SAU from pork sausages, pre-selected for some features for probiotic application, were utilized in this study to evaluate their adhesive properties and compare the results against the three pathogens also tested. Strains were tested for autoaggregation and coaggregation capacity and Microbial Adhesion To Solvents (MATS) at the time intervals of 0, 1, 2, 3 and 4 h. Our findings revealed that UFLA SAU strains have a high autoaggregative capacity and coaggregative ability with pathogens, especially Listeria monocytogenes. In relation to adhesion to solvents, in general, L. plantarum strains showed hydrophilic cell surface properties and an important electron donor and basic character. Adhesive properties were markedly separated for the strains under study by Principal Component Analysis software. UFLA SAU 132, 226 and 87 were differentiated by autoaggregation ability. UFLA SAU 11 and Listeria monocytogenes were characterized by adhesion to solvents. UFLA SAU 14, 18 and 172 showed high coaggregation with Escherichia coli, Salmonella Typhi and Listeria monocytogenes. In comparison to the pathogens tested, many UFLA SAU strains presented higher adhesive capacity. These tests should be used for screening and identifying potentially adherent microorganisms. Adhesive properties are important features for the choice of probiotic strains and confer various applications, such as in the pharmaceutical (therapeutic or prophylactic) and food (functional foods) industries.