Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/42180
metadata.artigo.dc.title: Potent neutralizing antibodies against SARS-CoV-2 identified by high-throughput single-cell sequencing of convalescent patients’ B cells
metadata.artigo.dc.creator: Cao, Yunlong
Su, Bin
Guo, Xianghua
Sun, Wenjie
Deng, Yongqiang
Bao, Linlin
Zhu, Qinyu
Zhang, Xu
Zheng, Yinghui
Geng, Chenyang
Chai, Xiaoran
He, Runsheng
Li, Xiaofeng
Lv, Qi
Zhu, Hua
Deng, Wei
Xu, Yanfeng
Wang, Yanjun
Qiao, Luxin
Tan, Yafang
Song, Liyang
Wang, Guopeng
Du, Xiaoxia
Gao, Ning
Liu, Jiangning
Xiao, Junyu
Su, Xiao-dong
Du, Zongmin
Feng, Yingmei
Qin, Chuan
Qin, Chengfeng
Jin, Ronghua
Xie, X. Sunney
metadata.artigo.dc.subject: Single-cell sequencing
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)
COVID-19
Neutralizing antibody
Convalescent patient
B cell
metadata.artigo.dc.publisher: Elsevier
metadata.artigo.dc.date.issued: Jul-2020
metadata.artigo.dc.identifier.citation: CAO, Y. et al. Potent neutralizing antibodies against SARS-CoV-2 identified by high-throughput single-cell sequencing of convalescent patients’ B cells. Cell, [S.l.], v. 182, n. 1, p. 73-84.e16, July 2020.
metadata.artigo.dc.description.abstract: The COVID-19 pandemic urgently needs therapeutic and prophylactic interventions. Here, we report the rapid identification of SARS-CoV-2-neutralizing antibodies by high-throughput single-cell RNA and VDJ sequencing of antigen-enriched B cells from 60 convalescent patients. From 8,558 antigen-binding IgG1+ clonotypes, 14 potent neutralizing antibodies were identified, with the most potent one, BD-368-2, exhibiting an IC50 of 1.2 and 15 ng/mL against pseudotyped and authentic SARS-CoV-2, respectively. BD-368-2 also displayed strong therapeutic and prophylactic efficacy in SARS-CoV-2-infected hACE2-transgenic mice. Additionally, the 3.8 Å cryo-EM structure of a neutralizing antibody in complex with the spike-ectodomain trimer revealed the antibody’s epitope overlaps with the ACE2 binding site. Moreover, we demonstrated that SARS-CoV-2-neutralizing antibodies could be directly selected based on similarities of their predicted CDR3H structures to those of SARS-CoV-neutralizing antibodies. Altogether, we showed that human neutralizing antibodies could be efficiently discovered by high-throughput single B cell sequencing in response to pandemic infectious diseases.
metadata.artigo.dc.identifier.uri: https://www.sciencedirect.com/science/article/pii/S0092867420306206
http://repositorio.ufla.br/jspui/handle/1/42180
metadata.artigo.dc.language: en_US
Appears in Collections:FCS - Artigos sobre Coronavirus Disease 2019 (COVID-19)

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.