Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/45962
Title: Hypothalamic stearoyl-CoA desaturase-2 (SCD2) controls whole-body energy expenditure
Keywords: Hypothalamus
Molecular neuroscience
Obesity
Physiology
Hipotálamo
Neurociência molecular
Obesidade
Fisiologia
Issue Date: 2016
Publisher: Springer Nature
Citation: MOURA, R. F. de et al. Hypothalamic stearoyl-CoA desaturase-2 (SCD2) controls whole-body energy expenditure. International Journal of Obesity, London, v. 40, p. 471-478, 2016.
Abstract: Background/Objectives: Stearoyl-CoA desaturase-2 (SCD2) is the main δ9 desaturase expressed in the central nervous system. Because of its potential involvement in controlling whole-body adiposity, we evaluated the expression and function of SCD2 in the hypothalami of mice. Subjects/Methods: Male mice of different strains were used in real-time PCR, immunoblot and metabolic experiments. In addition, antisense oligonucleotides and lentiviral vectors were used to reduce and increase the expression of SCD2 in the hypothalamus. Results: The level of SCD2 in the hypothalamus is similar to other regions of the central nervous system and is ~10-fold higher than in any other region of the body. In the arcuate nucleus, SCD2 is expressed in proopiomelanocortin and neuropeptide-Y neurons. Upon high fat feeding, the level of hypothalamic SCD2 increases. Inhibition of hypothalamic SCD2 as accomplished by two distinct approaches, an antisense oligonucleotide or a short-hairpin RNA delivered by a lentivirus, resulted in reduced body mass gain mostly due to increased energy expenditure and increased spontaneous activity. Increasing hypothalamic SCD2 by a lentivirus approach resulted in no change in body mass and food intake. Conclusions: Thus, SCD2 is highly expressed in the hypothalami of rodents and its knockdown reduces body mass due to increased whole-body energy expenditure.
URI: https://www.nature.com/articles/ijo2015188
http://repositorio.ufla.br/jspui/handle/1/45962
Appears in Collections:DME - Artigos publicados em periódicos

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