Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/56923
Title: Expression analysis of transcription factors in sugarcane during cold stress
Keywords: Environmental stress
Differentially expressed genes
Molecular reaction
Estresse ambiental
Genes diferencialmente expressos
Reação molecular
Issue Date: 2023
Publisher: Instituto Internacional de Ecologia
Citation: REHMAN, S. U. et al. Expression analysis of transcription factors in sugarcane during cold stress. Brazilian Journal of Biology, [S.l.], v. 83, 2023.
Abstract: Transcription factors (TF) are a wide class of genes in plants, and these can regulate the expression of other genes in response to various environmental stresses (biotic and abiotic). In the current study, transcription factor activity in sugarcane was examined during cold stress. Initially, RNA transcript reads of two sugarcane cultivars (ROC22 and GT08-1108) under cold stress were downloaded from SRA NCBI database. The reads were aligned into a reference genome and the differential expression analyses were performed with the R/Bioconductor edgeR package. Based on our analyses in the ROC22 cultivar, 963 TF genes were significantly upregulated under cold stress among a total of 5649 upregulated genes, while 293 TF genes were downregulated among a total of 3,289 downregulated genes. In the GT08-1108 cultivar, 974 TF genes were identified among 5,649 upregulated genes and 283 TF genes were found among 3,289 downregulated genes. Most transcription factors were annotated with GO categories related to protein binding, transcription factor binding, DNA-sequence-specific binding, transcription factor complex, transcription factor activity in RNA polymerase II, the activity of nucleic acid binding transcription factor, transcription corepressor activity, sequence-specific regulatory region, the activity of transcription factor of RNA polymerase II, transcription factor cofactor activity, transcription factor activity from plastid promoter, transcription factor activity from RNA polymerase I promoter, polymerase II and RNA polymerase III. The findings of above results will help to identify differentially expressed transcription factors during cold stress. It also provides a comprehensive analysis of the regulation of the transcription activity of many genes. Therefore, this study provides the molecular basis for improving cold tolerance in sugarcane and other economically important grasses.
URI: http://repositorio.ufla.br/jspui/handle/1/56923
Appears in Collections:DBI - Artigos publicados em periódicos

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