Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/57171
Title: Genetic diversity of Brucella ovis isolates from Rio Grande do Sul, Brazil, by MLVA16
Keywords: Brucella ovi
Ovine brucellosis
Brucelose ovina
Issue Date: 2014
Publisher: BioMed Central
Citation: DORNELES, E. M. S. et al. Genetic diversity of Brucella ovis isolates from Rio Grande do Sul, Brazil, by MLVA16. BMC Research Notes, [S. l.], v. 7, n. 447, p. 1-5, 2014.
Abstract: Background Ovine epididymitis is predominantly associated with Brucella ovis infection. Molecular characterization of Brucella spp. achieved by multi-locus variable number of tandem repeats (VNTR) analyses (MLVA) have proved to be a powerful tool for epidemiological trace-back studies. Thus, the aim of this study was to evaluate the genetic diversity of Brucella ovis isolates from Rio Grande do Sul State, Brazil, by MLVA16. Findings MLVA16 genotyping identified thirteen distinct genotypes and a Hunter-Gaston diversity index of 0.989 among the fourteen B. ovis genotyped strains. All B. ovis MLVA16 genotypes observed in the present study represented non-previously described profiles. Analyses of the eight conserved loci included in panel 1 (MLVA8) showed three different genotypes, two new and one already described for B. ovis isolates. Among ten B. ovis isolates from same herd only two strains had identical pattern, whereas the four isolates with no epidemiologic information exhibited a single MLVA16 pattern each. Analysis of minimal spanning tree, constructed using the fourteen B. ovis strains typed in this study together with all nineteen B. ovis MLVA16 genotypes available in the MLVAbank 2014, revealed the existence of two clearly distinct major clonal complexes. Conclusions In conclusion, the results of the present study showed a high genetic diversity among B. ovis field isolates from Rio Grande do Sul State, Brazil, by MLVA16.
URI: https://bmcresnotes.biomedcentral.com/articles/10.1186/1756-0500-7-447
http://repositorio.ufla.br/jspui/handle/1/57171
Appears in Collections:DMV - Artigos publicados em periódicos

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.