Indução in vitro de calos em explantes foliares de pequizeiro (Caryocar brasiliense Camb.)

Abstract

With the objective to determine a methodology for the in vitro establishment of pequizeiro, leaves were used as source of explantes for the induction of callus formation. The use ofWPM médium supplemented with the combinations of2.22 uM BAP + 10.74 uM NAA or 4.44 uM BAP + 10.74 uM NAA were able to induce callus formation on leaf explantes. Explantes maintained in the absence of hght showed higher callus formation. The formed callus presented white, yellowish or brown coloration. No formation of friable callus was observed The use ofbarley extract favored callus subculture which should be performed at each 45 days interval. The studies ofgenetic variability through RAPD indicated genetic proximity among the anahsed individuais suggesting that different explant behaviors observed in vitro were due to environmental and/orphysiological ratherthangenetic factors.