Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/11852
Title: Compostos orgânicos voláteis emitidos por Fusarium oxysporum apresentam atividade nematicida
Other Titles: Volatiles organic compounds emitted by Fusarium shows nematicidal activity
Authors: Campos, Vicente Paulo
Pfenning, Ludwig Heinrich
Pedroso, Marcio Pozzobon
Freire, Eduardo Souza
Salustiano, Maria Eloisa
Keywords: Nematóides de plantas
Fungos como agentes de controle da praga biológica
Compostos orgânicos
Plant nematodes
Fungi as biological pest control agents
Organic compounds
Fusarium oxysporum
Meloidogyne incognita
Heterodera schachtii
Issue Date: 3-Oct-2016
Publisher: Universidade Federal de Lavras
Citation: TERRA, W. C. Compostos orgânicos voláteis emitidos por Fusarium oxysporum apresentam atividade nematicida. 2016. 68 p. Tese (Doutorado em Agronomia/Fitopatologia)-Universidade Federal de Lavras, Lavras, 2016.
Abstract: A series of assays was carried out aiming to understand the nematicidal activity of the volatile organic compounds (VOCs) emitted by Fusarium oxysporum isolated 21 (Fo-21) on plant-parasitic nematodes. Through analysis by gas chromatography coupled to mass spectrometry (GC-MS) 50 compounds were detected in the Fo-21 emission. The main classes of compounds emitted by Fo-21 were sesquiterpenes, esters and alcohols. Water exposed to VOCs caused 100% immobility in second-stage juveniles (J 2) of Meloidogyne incognita. In addition, inoculation of those J2 in tomato decreased infectivity up to 70% and reproduction up to 65%. Application of water exposed to VOCs in substrate infested with M. incognita J 2 resulted in 56% reduction in number of galls and 49% in number of eggs. The eight most abundant VOCs emitted by F.o–21 were tested against M. incognita and Heterodera schachtii. Compounds 2– methylbutyl acetate (1), 3-methylbutyl acetate (2), ethyl acetate (7) and, 2-methylpropyl acetate (8), caused in vitro mortality of 100%, 92.5%, 100% and 83.8%, respectively, in M. incognita and 90.0%, 88.8%, 96.3% and 97.5% mortality, respectively, on H. schachtii J 2, when tested at a concentration of 500 µg.ml -1 . The lethal concentration (LC50) for compounds 1, 2, 7 and 8, in M. incognita J 2 was of 236, 198, 213 and 218 µg.ml -1 , respectively. Under the same conditions, the commercial nematicide carbofuran (2,3-dihydro-2,2-dimethyl-1-benzofuran-7-yl N-methylcarbamate) showed a LC50 of 191 µg.ml -1 . Eggs exposed to compounds 1, 2, 7 and 8, for 72 h showed a reduction in hatching of 36.5%, 55.2%, 91.0% and 10.3%, respectively, and reduction in infectivity of 53.0%, 53.0%, 32.0 % and 39.0%, respectively. When compounds were applied in tomato seedlings infested by M. incognita, compound 1 reduced the number of galls per gram of root in 27.5% when compared to the negative control (without the application of nematicide).
URI: http://repositorio.ufla.br/jspui/handle/1/11852
Appears in Collections:Agronomia/Fitopatologia - Doutorado (Teses)



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