Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/13449
Title: Diversidade de rizóbios isolados de diferentes sistemas de uso da terra na Amazônia
Authors: Moreira, Fátima M.S.
Siqueira, José Oswaldo
Carvalho , Dulcinéia de
Rumjanek, Norma Gouvéa
Straliotto, Rosângela
Keywords: Biodiversidade
Solo - Uso
Rizóbio
Feijão-de-corda
Feijão
Land use
Biodiversity
Rhizobium
Cowpea
Beans
Issue Date: 31-Jul-2017
Publisher: Universidade Federal de Lavras
Citation: PEREIRA, E. G. Diversidade de rizóbios isolados de diferentes sistemas de uso da terra na Amazônia. 2000. 93 p. Tese (Doutorado em Agronomia/Solos e Nutrição de Plantas)-Universidade Federal de Lavras, Lavras, 2000.
Abstract: Bèlow ground microbiota is thought to have a close relationship with above ground biodiversity. Besides, lost ofmicrobial diversity can lead to a lost of function reducing ecosystem sustaintability. BNF is among the most important soil functions, especially those performance by rhizobia symbiosis with legumes species. Aiming to evaluate densities as well as phenotypic, genetic and symbiotic diversity ofrhizobia populations at soil samples collected from three sites of each one ofthe following land use systems (LUS) in Amazon region: disturbed forest, fàllow, pasture, crops and agroforestry systems, greenhouse and laboratory assays were carried out. Siratro (Macroptilium atropurpureum Urb.) and cowpea (Vigna unguiculata (L.) Walp) were used as trap hosts for rhizobia strains, being the former also used to determine rhizobia most probable numbers and population efficiencies at the LUS. Rhizobia were also isolated from trap hosts and from non inoculated bean plants and other leguminous species which were found nodulatíng with native populations at some sites. Ali isolates were characterized culturally on YMA médium. Ali bean and cowpea isolates and some siratro isolates were characterized by polyacrilamide gel electrophoresis (SDS-PAGE) oftotal proteins. Genetic analysis ofbean isolates was made by comparison of restriction pattems with diflèrent enzymes to amplifíed 16S rRNA fragments (RFLP). At greenhouse conditions 26 cowpea isolates and 14 bean isolates were evaluated for the biological nitrogen fixation (BNF) efficiency to their homologous hosts. There was effect ofLUS on densities and efficiency ofrhizobia populations trapped by siratro. Bean isolates showed the same cultural characteristics on YMA but were highly mscriminated by SDSPAGE oftotal proteins and RFLP patterns. Most ofthem were highly efficient in BNF and even higher than recommended strain CIAT 899. Great phenotypic and symbiotic diversity was also found among cowpea isolates most of them showing superior efficiency to recommended strain BR 2001. The different LUS presented high diversity ofrhizobia populations. Cultural diversity calculated by Shannon-Weaver index decreased in the order: forest > agroforestry system> pasture> fallow > crops.
Description: Esta dissertação/tese está disponível online com base na Resolução CEPE nº 090, de 24 de março de 2015, disponível em http://www.biblioteca.ufla.br/wordpress/wp-content/uploads/res090-2015.pdf, que dispõe sobre a disponibilização da coleção retrospectiva de teses e dissertações online no Repositório Institucional da UFLA, sem autorização prévia dos autores. Parágrafo Único. Caberá ao autor ou orientador a solicitação de restrição quanto à divulgação de teses e dissertações com pedidos de patente ou qualquer embargo similar. Art. 5º A obra depositada no RIUFLA que tenha direitos autorais externos à Universidade Federal de Lavras poderá ser removida mediante solicitação por escrito, exclusivamente do autor, encaminhada à Comissão Técnica da Biblioteca Universitária./ Arquivo gerado por meio da digitalização de material impresso. Alguns caracteres podem ter sido reconhecidos erroneamente.
URI: http://repositorio.ufla.br/jspui/handle/1/13449
Appears in Collections:Ciência do Solo - Doutorado (Teses)



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