Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/1479
Título: Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seeds
Título(s) alternativo(s): Sensibilidade da técnica PCR na detecção de Stenocarpella sp. associados às sementes de milho
Palavras-chave: Fungus
Molecular detection
Stalk and ear rot
Macrospore leaf spot
Fungo
Detecção molecular
Podridão do colmo e da espiga
Mancha foliar de macrospora
Data do documento: 2012
Editor: Associação Brasileira de Tecnologia de Sementes
Citação: BARROCAS, E. N. et al. Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seeds. Revista Brasileira de Sementes, Londrina, v. 34, n. 2, p. 218-224, 2012.
Resumo: Maize seeds, infected by Stenocarpella species, are important sources of inoculum for the introduction and dissemination of stalk and ear rot and macrospore leaf spot diseases. The use of healthy seeds is an important strategy for the preventive control of these diseases. However, one of the difficulties in the health quality control programs for maize seeds is the availability of a reliable and quick method for detecting these fungi during routine seed analyses. Therefore, the objective of the present study was to investigate the possibility of using the PCR technique as an alternative method for accurately detecting these pathogens in maize seed samples. Maize seeds were kept in contact with S. maydis colonie developed in PDA media containing mannitol at -1.4 MPa for 72 h. The seed samples used in this study were prepared with infected seeds at incidences of 100, 20, 10, 2, 1 and zero %.The primers used were able to detect S. maydis fungi in association with seeds with a maximum of 2% , however those primers were not able to differentiate between the two species.
URI: http://repositorio.ufla.br/jspui/handle/1/1479
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