Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/28303
Title: Physiological quality and expression of genes in seeds of handroanthus serratifolius subjected to drying
Other Titles: Qualidade fisiológica e expressão de genes em sementes de Handroanthus serratifolius submetidas à secagem
Keywords: Isoenzymes
Enzymes
qRT-PCR
Handroanthus serratifolius
Isoenzimas
Enzimas
RTqPCR
Ipê-amarelo
Issue Date: 2015
Publisher: Associação Brasileira de Tecnologia de Sementes
Citation: GONÇALVES, L. H. do N. et al. Physiological quality and expression of genes in seeds of handroanthus serratifolius subjected to drying. Journal of Seed Science, Londrina, v. 37, n. 2, p. 102-110, 2015.
Abstract: Handroanthus serratifolius seeds are considered orthodox and have some mechanisms that assist in desiccation tolerance, like heat-resistant proteins and the presence of protective systems against free radical production. In order to investigate the drying effect on the quality of Handroanthus serratifolius seeds collected with 28% water content two methods were used: slow drying (30 °C) and fast drying (45 °C) until water contents equaled 20%, 15%, 10% and 5%. Seed quality was assessed by germination test, first count of germination, speed germination, emergence and speed emergence. The expression of heat-resistant proteins and enzymes catalase, esterase and isocitrate lyase was assessed by electrophoresis. The gene expression of sHSP17.5, CAT3 and ICL6 was quantified by real time PCR. Higher values of germination and vigor were obtained in seeds with 20% water content, subjected to fast drying. There was induction of heat-resistant proteins expression for both drying methods. There was variation in the electrophoretic pattern of catalase, esterase and isocitrate lyase in seeds subjected to different treatments. The gene expression in Handroanthus serratifolius seeds varies depending on methods of drying and water content of seeds. The drying of Handroanthus serratifolius seeds until the water content between 15 and 10% compromises their physiological quality.
URI: http://repositorio.ufla.br/jspui/handle/1/28303
Appears in Collections:DAG - Artigos publicados em periódicos



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