Please use this identifier to cite or link to this item:
|metadata.artigo.dc.title:||Relationship among physiological quality, heterosis, and amylase gene expression in maize seeds|
|metadata.artigo.dc.creator:||Oliveira, Gustavo Evangelista|
Von Pinho, Édila Vilela de Resende
Andrade, Thaís de
Souza, J. C.
Ferreira, R. A. D. C.
|metadata.artigo.dc.subject:||Quantitative real-time-polymerase chain reaction|
|metadata.artigo.dc.publisher:||Fundação de Pesquisas Científicas de Ribeirão Preto|
|metadata.artigo.dc.identifier.citation:||OLIVEIRA, G. E. et al. Relationship among physiological quality, heterosis, and amylase gene expression in maize seeds. Genetics and Molecular Research, Ribeirão Preto, v. 14, n. 3, p. 8623-8633, 2015.|
|metadata.artigo.dc.description.abstract:||In this study, we analyzed heterosis, amylase enzyme gene expression, and the physiological quality of maize seeds with different genotypes and sizes, which were subjected to aging and not subjected to aging. We used seeds from 2 maize lines that differed with regard to physiological quality, the hybrid, and the reciprocal hybrid; they were classified into 2 sizes and were subjected to aging and not subjected to aging. Physiological quality was assessed by performing tests for germination, emergence, emergence speed index, and artificial aging. Expressions of the genes alpha amylase B73, alpha amylase (LOC542522), isoamylase mRNA clone 353244, and the endogenous controls ubiquitin and alcohol dehydrogenase in the seeds were studied using quantitative real-time-polymerase chain reaction. We observed heterosis for seed quality and for expression of amylase genes in the genotypes studied. We found no difference in seed quality between large and small seeds.|
|Appears in Collections:||DAG - Artigos publicados em periódicos|
DBI - Artigos publicados em periódicos
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.