Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/28547
Title: Morphological characteristics and cell viability of coffee plants calli
Other Titles: Características morfológicas e viabilidade celular de calos de cafeeiro
Keywords: Coffea arabica
Electron microscopy
Tetrazolium test
Microscopia eletrônica
Teste do tetrazólio
Issue Date: Apr-2014
Publisher: Universidade Federal de Santa Maria
Citation: PÁDUA, M. S. et al. Morphological characteristics and cell viability of coffee plants calli. Ciência Rural, Santa Maria, v. 44, n. 4, p. 660-665, abr. 2014.
Abstract: The aim of this research was to characterize and compare two types of calli from leaf explants of Coffea arabica (cultivar Catiguá). Cells of different types of callus were successfully characterized regarding viability and internal and external morphological characteristics. It was obtained two morphologically distinct types of callus: (i) yellow friable and (ii) transparent watery. The yellow friable calli showed higher cell viability and embryogenic characteristics. Scanning and transmission electron microscopy showed embryogenic characteristics in cells of the yellow friable calli evidenced by the presence of small and isodiametric cells, while transparent watery calli showed elongated cells and large cytoplasm vacuolization.
URI: http://repositorio.ufla.br/jspui/handle/1/28547
Appears in Collections:DFP - Artigos publicados em periódicos
DQI - Artigos publicados em periódicos

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