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Title: | Morphological characteristics and cell viability of coffee plants calli |
Other Titles: | Características morfológicas e viabilidade celular de calos de cafeeiro |
Keywords: | Coffea arabica Electron microscopy Tetrazolium test Microscopia eletrônica Teste do tetrazólio |
Issue Date: | Apr-2014 |
Publisher: | Universidade Federal de Santa Maria |
Citation: | PÁDUA, M. S. et al. Morphological characteristics and cell viability of coffee plants calli. Ciência Rural, Santa Maria, v. 44, n. 4, p. 660-665, abr. 2014. |
Abstract: | The aim of this research was to characterize and compare two types of calli from leaf explants of Coffea arabica (cultivar Catiguá). Cells of different types of callus were successfully characterized regarding viability and internal and external morphological characteristics. It was obtained two morphologically distinct types of callus: (i) yellow friable and (ii) transparent watery. The yellow friable calli showed higher cell viability and embryogenic characteristics. Scanning and transmission electron microscopy showed embryogenic characteristics in cells of the yellow friable calli evidenced by the presence of small and isodiametric cells, while transparent watery calli showed elongated cells and large cytoplasm vacuolization. |
URI: | http://repositorio.ufla.br/jspui/handle/1/28547 |
Appears in Collections: | DFP - Artigos publicados em periódicos DQI - Artigos publicados em periódicos |
Files in This Item:
File | Description | Size | Format | |
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ARTIGO_Morphological characteristics and cell viability of coffee plants calli.pdf | 768,97 kB | Adobe PDF | View/Open |
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