Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/28713
metadata.artigo.dc.title: Spore behaviors reveal a category of mating-competent infertile heterokaryons in the offspring of the medicinal fungus Agaricus subrufescens
metadata.artigo.dc.creator: Brito, Manuela Rocha de
Foulongne-Oriol, Marie
Moinard, Magalie
Dias, Eustáquio Souza
Savoie, Jean-Michel
Callac, Philippe
metadata.artigo.dc.subject: Mushroom
Pseudohomothallism
Buller phenomenon
Life cycle
Breeding program
metadata.artigo.dc.publisher: Springer
metadata.artigo.dc.date.issued: Jan-2016
metadata.artigo.dc.identifier.citation: BRITO, M. R. de et al. Spore behaviors reveal a category of mating-competent infertile heterokaryons in the offspring of the medicinal fungus Agaricus subrufescens. Applied Microbiology and Biotechnology, Berlin, v. 100, n. 2, p. 781–796, Jan. 2016.
metadata.artigo.dc.description.abstract: Strain breeding is much less advanced in the edible and medicinal species Agaricus subrufescens than in Agaricus bisporus, the button mushroom. Both species have a unifactorial system of sexual incompatibility, a mating type locus tightly linked to a centromere, and basidia producing both homokaryotic (n) and heterokaryotic (n + n) spores. In A. bisporus, breeding is mainly based on direct selection among the heterokaryotic offspring and on hybridization between homokaryotic offspring. The parental heterozygosity is highly maintained in the heterokaryotic offspring due to suppression of recombination and preferential pairing in the spores of nuclei, each one per second meiotic divisions; such “non-sister nuclei” heterokaryons are fertile. In A. subrufescens, recent studies revealed that recombination is not suppressed and that nuclei from the same second meiotic division can also be paired in a spore that give rise to a “sister nuclei” heterokaryon in which the nuclei bear the same mating type allele. The objective of the present work was to investigate the potential function of the different categories of spores in A. subrufescens and their possible use in a genetic breeding program. Using eight co-dominant molecular markers, we found that half of the offspring of the A. subrufescens strain WC837 were heterokaryotic, one quarter of them being sister nuclei heterokaryons. These heterokaryons were infertile and behaved like homokaryons, being even able to cross between each other. In contrast, non-sister nuclei heterokaryons could fruit but inconsistently due to inbreeding depression. Potential roles of these two categories of heterokaryons in nature and consequences for strain breeding are discussed.
metadata.artigo.dc.identifier.uri: https://link.springer.com/article/10.1007/s00253-015-7070-7
http://repositorio.ufla.br/jspui/handle/1/28713
metadata.artigo.dc.language: en_US
Appears in Collections:DBI - Artigos publicados em periódicos

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